Development and testing of novel chloroplast microsatellite markers for Lolium perenne and other grasses (Poaceae) from de novo sequencing and in silico sequences

被引：14

作者：

Mcgrath, S.

Hodkinson, T. R.

Salamin, N.

Barth, S. ^{[1
]}

机构：

[1] TEAGASC, Crops Res Ctr, Carlow, Ireland

[2] Univ Dublin, Trinity Coll, Sch Nat Sci, Dept Bot, Dublin 2, Ireland

[3] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 02期

关键词：

chloroplast microsatellites; Lolium perenne; plastid; Poaceae; SSRs; transferability;

D O I：

10.1111/j.1471-8286.2006.01267.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Twelve primers to amplify microsatellite markers from the chloroplast genome of Lolium perenne were designed and optimized using de novo sequencing and in silico sequences. With one exception, each locus was polymorphic with a range from two to nine alleles in L. perenne. The newly developed primer pairs cross-amplified in different species of Lolium and in 50 other grass species representing nine grass subfamilies.

引用

页码：449 / 452

页数：4