Tumor Suppressive Function of mir-205 in Breast Cancer Is Linked to HMGB3 Regulation

被引:98
|
作者
Elgamal, Ola A. [1 ]
Park, Jong-Kook [1 ]
Gusev, Yuriy [2 ]
Azevedo-Pouly, Ana Clara P. [1 ]
Jiang, Jinmai [1 ]
Roopra, Avtar [3 ]
Schmittgen, Thomas D. [1 ]
机构
[1] Ohio State Univ, Coll Pharm, Columbus, OH 43210 USA
[2] Georgetown Univ, Ctr Canc, Washington, DC USA
[3] Univ Wisconsin, Dept Neurosci, Madison, WI USA
来源
PLOS ONE | 2013年 / 8卷 / 10期
关键词
EXPRESSION ANALYSIS; LUNG-CANCER; MICRORNA-205; MELANOMA; CARCINOMAS; PROGNOSIS; PROFILES; PROSTATE; PROTEIN; FAMILY;
D O I
10.1371/journal.pone.0076402
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Identifying targets of dysregulated microRNAs (miRNAs) will enhance our understanding of how altered miRNA expression contributes to the malignant phenotype of breast cancer. The expression of miR-205 was reduced in four breast cancer cell lines compared to the normal-like epithelial cell line MCF10A and in tumor and metastatic tissues compared to adjacent benign breast tissue. Two predicted binding sites for miR-205 were identified in the 3' untranslated region of the high mobility group box 3 gene, HMGB3. Both dual-luciferase reporter assay and Western blotting confirmed that miR-205 binds to and regulates HMGB3. To further explore miR-205 targeting of HMGB3, WST-1 proliferation and in vitro invasion assays were performed in MDA-MB-231 and BT549 cells transiently transfected with precursor miR-205 oligonucleotide or HMGB3 small interfering RNA (siRNA). Both treatments reduced the proliferation and invasion of the cancer cells. The mRNA and protein levels of HMGB3 were higher in the tumor compared to adjacent benign specimens and there was an indirect correlation between the expression of HMGB3 mRNA and patient survival. Treatment of breast cancer cells with 5-Aza/TSA derepressed miR-205 and reduced HMGB3 mRNA while knockdown of the transcriptional repressor NRSF/REST, reduced miR-205 and increased HMGB3. In conclusion, regulation of HMGB3 by miR-205 reduced both proliferation and invasion of breast cancer cells. Our findings suggest that modulating miR-205 and/or targeting HMGB3 are potential therapies for advanced breast cancer.
引用
收藏
页数:12
相关论文
共 50 条
  • [21] miR-205 is frequently downregulated in prostate cancer and acts as a tumor suppressor by inhibiting tumor growth
    Wang, Ning
    Li, Qi
    Feng, Ning-Han
    Cheng, Gong
    Guan, Zhao-Long
    Wang, Yang
    Qin, Chao
    Yin, Chang-Jun
    Hua, Li-Xin
    ASIAN JOURNAL OF ANDROLOGY, 2013, 15 (06) : 735 - 741
  • [22] MIR-205 SUPPRESSES TUMOR GROWTH AND PROGRESSION IN CASTRATION-RESISTANT PROSTATE CANCER
    Ning, W.
    Yang, W.
    Ning, S.
    Qi, L.
    Ninghan, F.
    INTERNATIONAL JOURNAL OF UROLOGY, 2012, 19 : 281 - 281
  • [23] MicroRNA expression profiling identifies decreased expression of miR-205 in inflammatory breast cancer
    Huo, Lei
    Wang, Yan
    Gong, Yun
    Krishnamurthy, Savitri
    Wang, Jing
    Diao, Lixia
    Liu, Chang-Gong
    Liu, Xiuping
    Lin, Feng
    Symmans, William F.
    Wei, Wei
    Zhang, Xinna
    Sun, Li
    Alvarez, Ricardo H.
    Ueno, Naoto T.
    Fouad, Tamer M.
    Harano, Kenichi
    Debeb, Bisrat G.
    Wu, Yun
    Reuben, James
    Cristofanilli, Massimo
    Zuo, Zhuang
    MODERN PATHOLOGY, 2016, 29 (04) : 330 - 346
  • [24] Dual function miR-205 is positively associated with ER and negatively with five-year survival in breast cancer patients
    Petrovic, Nina
    Todorovic, Lidija
    Nedeljkovic, Milica
    Bozovic, Ana
    Bukumiric, Zoran
    Tanic, Nasta Dedovic
    Jovanovic-Cupic, Snezana
    Sami, Ahmad
    Mandusic, Vesna
    PATHOLOGY RESEARCH AND PRACTICE, 2022, 238
  • [25] miR-145, miR-205 and miR-451: potential tumor suppressors involved in the progression of in situ to invasive carcinoma of the breast
    Ji Won Woo
    Hye Yeon Choi
    Milim Kim
    Yul Ri Chung
    So Yeon Park
    Breast Cancer, 2022, 29 : 814 - 824
  • [26] Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer
    Piovan, Claudia
    Palmieri, Dario
    Di Leva, Gianpiero
    Braccioli, Luca
    Casalini, Patrizia
    Nuovo, Gerard
    Tortoreto, Monica
    Sasso, Marianna
    Plantamura, Ilaria
    Triulzi, Tiziana
    Taccioli, Cristian
    Tagliabue, Elda
    Iorio, Marilena V.
    Croce, Carlo M.
    MOLECULAR ONCOLOGY, 2012, 6 (04) : 458 - 472
  • [27] miR-145, miR-205 and miR-451: potential tumor suppressors involved in the progression of in situ to invasive carcinoma of the breast
    Woo, Ji Won
    Choi, Hye Yeon
    Kim, Milim
    Chung, Yul Ri
    Park, So Yeon
    BREAST CANCER, 2022, 29 (05) : 814 - 824
  • [28] THERAPEUTIC POTENTIAL OF LET-7, MIR-125, MIR-205, AND MIR-296 IN BREAST CANCER: AN UPDATE
    Barh, Debmalya
    Vedamurthy, A. B.
    IIOAB JOURNAL, 2013, 4 (01) : 27 - 28
  • [29] Correction to: miR-145, miR-205 and miR-451: potential tumor suppressors involved in the progression of in situ to invasive carcinoma of the breast
    Ji Won Woo
    Hye Yeon Choi
    Milim Kim
    Yul Ri Chung
    So Yeon Park
    Breast Cancer, 2023, 30 : 156 - 156
  • [30] The effect of kinase signaling for miR-205 regulation in gefitinib-resistant lung cancer cell lines
    Suzuki, Toshihiro
    Nagasawa, Ikuko
    Yamaoka, Toshimitsu
    Ohmori, Tohru
    Nishio, Kazuto
    Koyama, Kiyotaka
    Ogasawara, Yuki
    CANCER RESEARCH, 2016, 76