Design and Synthesis of Novel Pyrazolo[3,4-d]Pyrimidines:In VitroCytotoxic Evaluation and Free Radical Scavenging Activity Studies

With evident biological importance, a new series of pyrazolo[3,4-d]pyrimidines3a,3band4a,4bwere synthesized via the formation of pyrazol-3-one2aand2bsubstrates. All compounds were evaluated forin vitrocytotoxic activity against MCF-7 (breast adenocarcinoma) and A549 (lung cancer) cell lines. The obtained results showed that pyrazolo[3,4-d] pyrimidin-4-ol3abearing phenyl group atN-1 andp-C(6)H(4)atC-6, and4bwith dinitrophenyl atN-1 and furanyl moiety atC-6 had better inhibitory activity against MCF-7 with IC(50)values in a micromolar range as compared to other substrates. The synthesized compounds can be considered as new candidates for further optimization as anticancer agents.