Tissue Factor Pathway Inhibitor-2 Silencing Promotes Hepatocellular Carcinoma Cell Invasion In Vitro

被引:19
|
作者
Zhu, Bin [1 ]
Zhang, Ping [1 ]
Zeng, Ping [1 ]
Huang, Zhen [1 ]
Dong, Ting-Fang [1 ]
Gui, Yong-Kun [1 ]
Zhang, Guang-Wen [1 ]
机构
[1] Xinxiang Med Univ, Affiliated Hosp 1, Dept Infect Dis, Xinxiang 453100, Peoples R China
来源
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2013年 / 296卷 / 11期
关键词
tissue factor pathway inhibitor-2; hepatocellular carcinoma; invasion; CANCER; METASTASIS; ANGIOGENESIS; INVOLVEMENT; CD44;
D O I
10.1002/ar.22789
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death in the world and metastasis is an essential aspect of HCC progression. Tissue factor pathway inhibitor-2 (TFPI-2) has been implicated as a potential suppressor gene to regulate tumor invasion and metastasis. In this study, we silenced TFPI-2 in the HCC cell line MHCC97-L and evaluated the role of TFPI-2 in cell invasion and its impact on gene expression. We showed in this study that stable TFPI-2 downregulation in MHCC97-L cells resulted in increased cell adhesion and invasion. We also showed that mRNA and protein expression levels of MMP-1/3, CD44, and ICAM-1 were increased, while those of MMP-2/9 were not changed by TFPI-2 silencing. Furthermore, silencing of TFPI-2 caused increased Akt phosphorylation level and NF-B transcription in MHCC97-L cells. In conclusion, this study confirms that TFPI-2 downregulation can contribute to tumor invasion of HCC cells through alteration in the expression of metastasis-related genes. Anat Rec, 296:1708-1716, 2013. (c) 2013 Wiley Periodicals, Inc.
引用
收藏
页码:1708 / 1716
页数:9
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