Single-molecule DNA detection using a novel SP1 protein nanopore

被引：33
|
作者：
Wang, Hai-Yan [1 ,2 ,3 ]
Li, Yang [1 ,2 ,3 ]
Qin, Li-Xia [1 ,2 ,3 ]
Heyman, Arnon [4 ]
Shoseyov, Oded [4 ]
Willner, Itamar [5 ]
Long, Yi-Tao [1 ,2 ,3 ]
Tian, He [1 ,2 ,3 ]
机构：
[1] E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China
[2] E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China
[3] E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China
[4] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet Agr, Fac Agr Food & Environm Qual Sci, Jerusalem, Israel
[5] Hebrew Univ Jerusalem, Inst Chem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 17期
基金：
中国国家自然科学基金; 上海市自然科学基金;
关键词：
ALPHA-HEMOLYSIN; DISCRIMINATION; TRANSLOCATION;
D O I：
10.1039/c3cc38939a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
SP1 protein as a new type of biological nanopore is described and is utilized to distinguish single-stranded DNA at the single-molecule level. Using the SP1 nanopore to investigate single molecule detection broadens the existing research areas of pore-forming biomaterials from unsymmetrical biological nanopores to symmetrical biological nanopores. This novel nanopore could provide a good candidate for single-molecule detection and characterization of biomaterial applications.
引用
收藏
页码：1741 / 1743
页数：3
相关论文
共 50 条
  • [21] Biological Nanopore Approach for Single-Molecule Protein Sequencing
    Hu, Zheng-Li
    Huo, Ming-Zhu
    Ying, Yi-Lun
    Long, Yi-Tao
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 60 (27) : 14738 - 14749
  • [22] Nanopore single-molecule biosensor in protein denaturation analysis
    Sun, Hong
    Yao, Chuan
    You, Kaibo
    Chen, Can
    Liu, Shuoshuo
    Xu, Zhihong
    ANALYTICA CHIMICA ACTA, 2023, 1243
  • [23] Alpha-hederin nanopore for single-molecule detection
    Jeong, Kibaek
    Luo, Ke
    Kim, Young-Rok
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 258
  • [24] Nanopore Unzipping Of Ultra-long Dna Repeats For Single-molecule Mutation Detection
    Yu, Zhiliang
    McNally, Ben
    Meller, Amit
    BIOPHYSICAL JOURNAL, 2009, 96 (03) : 645A - 645A
  • [25] DNA computing using single-molecule hybridization detection
    Schmidt, KA
    Henkel, CV
    Rozenberg, G
    Spaink, HP
    NUCLEIC ACIDS RESEARCH, 2004, 32 (17) : 4962 - 4968
  • [26] Measuring Single-Molecule DNA Hybridization by Active Control of DNA in a Nanopore
    Gyarfas, Brett
    Abu-Shumays, Robin
    Wang, Hongyun
    Dunbar, William B.
    BIOPHYSICAL JOURNAL, 2011, 100 (06) : 1509 - 1516
  • [27] Single-molecule nanopore enzymology
    Willems, Kherim
    Van Meervelt, Veerle
    Wloka, Carsten
    Maglia, Giovanni
    PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2017, 372 (1726)
  • [28] Direct detection of RNA modifications and structure using single-molecule nanopore sequencing
    Stephenson, William
    Razaghi, Roham
    Busan, Steven
    Weeks, Kevin M.
    Timp, Winston
    Smibert, Peter
    CELL GENOMICS, 2022, 2 (02):
  • [29] Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore Arrays
    McNally, Ben
    Singer, Alon
    Yu, Zhiliang
    Sun, Yingjie
    Weng, Zhiping
    Meller, Amit
    NANO LETTERS, 2010, 10 (06) : 2237 - 2244
  • [30] Nanopore immobilization of DNA polymerase enhances single-molecule sequencing
    Larkin, Joseph
    Foquet, Matieu
    Korlach, Jonas
    Wanunu, Meni
    JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2013, 31 : 134 - 135
12345