Simultaneous Trichothecenes Analysis by LC-MS/MS with a Pentafluorophenyl Column

被引:7
|
作者
Tamura, Masayoshi [1 ]
Nakagawa, Hiroyuki [2 ]
Uyama, Atsuo [1 ]
Mochizuki, Naoki [1 ]
机构
[1] Asahi Grp Holdings Ltd, Res Labs Food Safety Chem, Ibaraki 3020106, Japan
[2] Natl Agr & Food Res Org, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan
来源
FOOD HYGIENE AND SAFETY SCIENCE | 2014年 / 55卷 / 01期
关键词
Ztrichothecenes; pentafluorophenyl column; LC-MS/MS; 3-acetyldeoxynivalenol; 15-acetyldeoxynivalenol; QuEChERS extraction; TANDEM MASS-SPECTROMETRY; DEOXYNIVALENOL;
D O I
10.3358/shokueishi.55.19
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
We developed a method for simultaneous analysis of nine trichothecenes (nivalenol, deoxynivalenol, fusarenon-X, neosolaniol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, HT-2 toxin, and T-2 toxin). Trichothecenes in samples were extracted with acetonitrile-water (1: 1) and purified using a QuEChERS kit and a multi-functional cartridge. Then they were quantified by LC-MS/MS using internal standards. LC with a pentafluorophenyl column and a mobile phase containing methanol enabled complete separation and highly sensitive quantification of the nine trichothecenes. Method validation with powdered corn soup samples demonstrated high linearity (< 0.99) and accuracy ranging from 95% to 111% with good repeatability of 0.9% to 6.6% and reproducibility of 0.6% to 11.6%. The limits of detection and quantification were 0.01 to 0.75 fig/kg and 0.04 to 2.50 p.g/kg, respectively. When 15 samples from powdered corn soups on the market were analyzed with this method, deoxynivalenol was detected in two samples (20.7 and 22.5 mu g/kg, respectively), and 3-acetyldeoxynivalenol was concomitantly detected in one of them (13.5 mu g/kg).
引用
收藏
页码:19 / 24
页数:6
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