Regeneration and transformation studies in Terminalia chebula Retz.

This study presents in vitro regeneration of Terminalia chebula Retz. to obtain complete plantlets from juvenile explants (hypocotyl and cotyledon). Dried seeds were inoculated on MS basal medium after surface sterilization with Bavistin (0.2%) alone and followed by HgCl2 (0.1%), resulted in maximum (75%) germination. Hypocotyl showed 90% and cotyledon 75% callus induction on MS basal medium containing 1.0 mg/l 2, 4-D after 30 days of inoculation. Shoot regeneration was recorded only from cotyledonary callus on shoot induction medium comprising 1.5 mg/l BAP with 0.10 mg/l NAA with maximum 36.67% shoot regeneration. Maximum (43.75%) rooting was reported in 1/2 strength MS medium with 0.5% activated charcoal. Transgenic callus was produced through Agrobacterium tumefaciens mediated genetic transformation carrying gus and npt-II gene from cotyledonary explants. Co-cultivation (72 h) preceded by pre-conditioning (72 h) was found best for callus induction. Successful integration of gus gene was reported.