A HIGHLY SENSITIVE ELECTROCHEMICAL IMMUNOSENSOR FOR THE RAPID DETECTION OF TRIS(2,3-DIBROMOPROPYL) ISOCYANURATE

被引：3
|
作者：
Huang, Chen'an [1 ]
Zhou, Liping [1 ]
Li, Jiezhen [1 ]
Chen, Lan [1 ]
Yuan, Lijuan [1 ]
Shi, Lei [1 ]
Cai, Qingyun [1 ]
机构：
[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China
来源：
ANALYTICAL LETTERS | 2014年 / 47卷 / 05期
基金：
美国国家科学基金会;
关键词：
Competitive immunoassay; Electrochemical immunosensor; Horseradish peroxidase; Tris(2; 3-dibromopropyl) isocyanurate; BROMINATED FLAME RETARDANTS; POLYBROMINATED DIPHENYL ETHERS; STAPHYLOCOCCAL-ENTEROTOXIN-B; HEXABROMOCYCLODODECANES; HAPTEN; ASSAY;
D O I：
10.1080/00032719.2013.831432
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An electrochemical immunosensor was constructed for the sensitive determination of tris(2,3-dibromopropyl) isocyanurate. The analyte was first conjugated with ovalbumin. The conjugates were immobilized on the electrode surface through a chitosan membrane to compete with tris(2,3-dibromopropyl) isocyanurate in solution for specific immunoreaction with an antibody, followed by the immobilization of horseradish peroxidase-labeled secondary antibody. Finally, with the addition of hydrogen peroxide and hydroquinone, changes in the cathodic current were recorded. Under optimal conditions, tris(2,3-dibromopropyl) isocyanurate was determined in the range from 0.01 to 1M with a correlation coefficient of 0.9956, and a detection limit of 8.6nM. The immunosensor exhibited good precision, sensitivity, selectivity, and storage stability.
引用
收藏
页码：778 / 794
页数：17
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