A HIGHLY SENSITIVE ELECTROCHEMICAL IMMUNOSENSOR FOR THE RAPID DETECTION OF TRIS(2,3-DIBROMOPROPYL) ISOCYANURATE

被引：3

作者：

Huang, Chen'an ^{[1
]}

Zhou, Liping ^{[1
]}

Li, Jiezhen ^{[1
]}

Chen, Lan ^{[1
]}

Yuan, Lijuan ^{[1
]}

Shi, Lei ^{[1
]}

Cai, Qingyun ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

来源：

ANALYTICAL LETTERS | 2014年 / 47卷 / 05期

基金：

美国国家科学基金会;

关键词：

Competitive immunoassay; Electrochemical immunosensor; Horseradish peroxidase; Tris(2; 3-dibromopropyl) isocyanurate; BROMINATED FLAME RETARDANTS; POLYBROMINATED DIPHENYL ETHERS; STAPHYLOCOCCAL-ENTEROTOXIN-B; HEXABROMOCYCLODODECANES; HAPTEN; ASSAY;

D O I：

10.1080/00032719.2013.831432

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical immunosensor was constructed for the sensitive determination of tris(2,3-dibromopropyl) isocyanurate. The analyte was first conjugated with ovalbumin. The conjugates were immobilized on the electrode surface through a chitosan membrane to compete with tris(2,3-dibromopropyl) isocyanurate in solution for specific immunoreaction with an antibody, followed by the immobilization of horseradish peroxidase-labeled secondary antibody. Finally, with the addition of hydrogen peroxide and hydroquinone, changes in the cathodic current were recorded. Under optimal conditions, tris(2,3-dibromopropyl) isocyanurate was determined in the range from 0.01 to 1M with a correlation coefficient of 0.9956, and a detection limit of 8.6nM. The immunosensor exhibited good precision, sensitivity, selectivity, and storage stability.

引用

页码：778 / 794

页数：17

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