Changes in glycosaminoglycans and proteoglycans of normal breast and fibroadenoma during the menstrual cycle

被引:17
|
作者
de Lima, Cilene Reboucas [1 ]
dos Santos Junior, Jose de Arimatea [2 ]
Pinto Nazario, Afonso Celso [2 ]
Michelacci, Yara M. [1 ]
机构
[1] Univ Fed Sao Paulo UNIFESP, Dept Bioquim, Escola Paulista Med, Disciplina Biol Mol, BR-04044020 Sao Paulo, Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Ginecol, Escola Paulista Med, BR-04044020 Sao Paulo, Brazil
来源
基金
巴西圣保罗研究基金会;
关键词
Fibroadenoma; Proteoglycan; Menstrual cycle; Normal breast; Decorin; Versican; GROWTH-FACTOR-RECEPTOR; DERMATAN SULFATE; EXTRACELLULAR-MATRIX; CHONDROITIN SULFATE; FLAVOBACTERIUM-HEPARINUM; HEPARAN-SULFATE; CELL-GROWTH; FACTOR-BETA; DECORIN; EXPRESSION;
D O I
10.1016/j.bbagen.2012.04.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Fibroadenoma is the most common breast tumor in young women, and its growth and metabolism may be under hormonal control. In the present paper we described the proteoglycan (PG) composition and synthesis rate of normal breast and fibroadenoma during the menstrual cycle. Methods: Samples of fibroadenoma and adjacent normal breast tissue were obtained at surgery. PGs were characterized by agarose gel electrophoresis and enzymatic degradation with glycosaminoglycan (GAG) lyases, and immunolocalized by confocal microscopy. To assess the synthesis rate, PGs were metabolic labeled by S-35-sulfate. Results: The concentration of PGs in normal breast was higher during the secretory phase. Fibroadenoma contained and synthesized more PGs than their paired controls, but the PG concentrations varied less with the menstrual cycle and, in contrast to normal tissue, peaked in the proliferative phase. The main mammary GAGs are heparan sulfate (HS, 71%-74%) and dermatan sulfate (DS, 26%-29%). The concentrations of both increased in fibroadenoma, but DS increased more, becoming 35%-37% of total. The DS chains contained more beta-D-glucuronic acid (IdoUA/GlcUA ratios were >10 in normal breast and 2-7 in fibroadenoma). The S-35-sulfate incorporation rate revealed that the in vitro synthesis rate of DS was higher than HS. Decorin was present in both tissues, while versican was found only in fibroadenoma. Conclusions: In normal breast, the PG concentration varied with the menstrual cycle. It was increased in fibroadenoma, especially DS. General significance PGs are increased in fibroadenoma, but their concentrations may be less sensitive to hormonal control. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:1009 / 1019
页数:11
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