Ca2+ Signaling and Cell Death Induced by Protriptyline in HepG2 Human Hepatoma Cells

被引：1

作者：

Wang, Jue-Long ^{[1
]}

Chou, Chiang-Ting ^{[2
,3
]}

Liu, Kang ^{[4
]}

Liang, Wei-Zhe ^{[5
]}

Cheng, Jin-Shiung ^{[6
]}

Chang, Hong-Tai ^{[7
]}

Chen, I-Shu ^{[7
]}

Lu, Ti ^{[8
]}

Kuo, Chun-Chi ^{[9
]}

Yu, Chia-Cheng ^{[7
]}

Shieh, Pochuen ^{[10
]}

Kuo, Daih-Huang ^{[10
]}

Chen, Fu-An ^{[10
]}

Jan, Chung-Ren ^{[5
]}

机构：

[1] Kaohsiung Vet Gen Hosp, Dept Rehabil, Tainan Branch, Tainan, Taiwan

[2] Chang Gung Inst Technol, Div Basic Med Sci, Dept Nursing, Chiayi, Taiwan

[3] Chang Gung Inst Technol, Chron Dis & Hlth Promot Res Ctr, Chiayi, Taiwan

[4] Kaohsiung Vet Gen Hosp, Dept Anesthesia, Tainan Branch, Tainan, Taiwan

[5] Kaohsiung Vet Gen Hosp, Dept Med Educ & Res, Kaohsiung, Taiwan

[6] Kaohsiung Vet Gen Hosp, Dept Med, Kaohsiung, Taiwan

[7] Kaohsiung Vet Gen Hosp, Dept Surg, Kaohsiung, Taiwan

[8] Kaohsiung Vet Gen Hosp, Dept Psychiat, Kaohsiung 813, Taiwan

[9] Tzu Hui Inst Technol, Dept Nursing, Pingtung, Taiwan

[10] Tajen Univ, Dept Pharm, Pingtung, Taiwan

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2016年 / 30卷 / 11期

关键词：

Ca2+; Cytotoxicity; Human Hepatoma Cells; Phospholipase C; Protriptyline; PROSTATE-CANCER CELLS; OBSTRUCTIVE PULMONARY-DISEASE; PROTEIN-COUPLED RECEPTORS; CALCIUM-ENTRY; TRICYCLIC ANTIDEPRESSANTS; ENDOPLASMIC-RETICULUM; NEUROBLASTOMA-CELLS; PLASMA-LEVELS; IN-VITRO; APOPTOSIS;

D O I：

10.1002/jbt.21820

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of protriptyline on Ca2+ physiology in human hepatoma is unclear. This study explored the effect of protriptyline on [Ca2+](i) and cytotoxicity in HepG2 human hepatoma cells. Protriptyline (50-150M) evoked [Ca2+](i) rises. The Ca2+ entry was inhibited by removal of Ca2+. Protriptyline-induced Ca2+ entry was confirmed by Mn2+-induced quench of fura-2 fluorescence. Except nifedipine, econazole, SKF96365, GF109203X, and phorbol 12-myristate 13 acetate did not inhibit Ca2+ entry. Treatment with the endoplasmic reticulum Ca2+ pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) inhibited 40% of protriptyline-induced response. Treatment with protriptyline abolished BHQ-induced response. Inhibition of phospholipase C (PLC) suppressed protriptyline-evoked response by 70%. At 20-40M, protriptyline killed cells which was not reversed by the Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid-acetoxymethyl ester (BAPTA/AM). Together, in HepG2 cells, protriptyline induced [Ca2+](i) rises that involved Ca2+ entry through nifedipine-sensitive Ca2+ channels and PLC-dependent Ca2+ release from endoplasmic reticulum. Protriptyline induced Ca2+-independent cell death.

引用

页码：539 / 547

页数：9

共 50 条

[21] Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells
Chen, Chuan
Han, Zhu
Yang, Minjie
Jiang, Zhongke
Ou, Xiuyuan
MEDICINE, 2020, 99 (17) : E19848
[22] Anticancer effects of pyocyanin on HepG2 human hepatoma cells
Zhao, J.
Wu, Y.
Alfred, A. T.
Wei, P.
Yang, S.
LETTERS IN APPLIED MICROBIOLOGY, 2014, 58 (06) : 541 - 548
[23] Metabolism of petrogenic PAHs in human hepatoma (HepG2) cells
Huang, Meng
Blair, Ian A.
Penning, Trevor M.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012, 244
[24] The transcriptional signature of dioxin in human hepatoma HepG2 cells
Puga, A
Maier, A
Medvedovic, M
BIOCHEMICAL PHARMACOLOGY, 2000, 60 (08) : 1129 - 1142
[25] Characterization and modulation of LP(a) in human hepatoma HEPG2 cells
Vu, H
Cianflone, K
Zhang, ZJ
Kalant, D
Sniderman, AD
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1997, 1349 (02): : 97 - 108
[26] Involvement of catalase in the apoptotic mechanism induced by apigenin in HepG2 human hepatoma cells
Valdameri, Glaucio
Trombetta-Lima, Marina
Worfel, Paulo R.
Pires, Amanda R. A.
Martinez, Glaucia R.
Noleto, Guilhermina R.
Cadena, Silvia M. S. C.
Sogayar, Mari C.
Winnischofer, Sheila M. B.
Rocha, Maria E. M.
CHEMICO-BIOLOGICAL INTERACTIONS, 2011, 193 (02) : 180 - 189
[27] Enhancement of esculetin on Taxol-induced apoptosis in human hepatoma HepG2 cells
Kuo, HC
Lee, HJ
Hu, CC
Shun, HI
Tseng, TH
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2006, 210 (1-2) : 55 - 62
[28] Streptozotocin-induced cytotoxicity and mitochondrial dysfunction in human hepatoma HepG2 cells
Raza, Haider
John, Annie
CANCER RESEARCH, 2010, 70
[29] Cytotoxicity and apoptosis induced by a new podophyllotoxin glucoside in human hepatoma (HepG2) cells
Liu, Yanfeng
Zhao, Changqi
Li, Hongxing
Yu, Miao
Gao, Jiali
Wang, Lei
Zhai, Yonggong
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2010, 88 (04) : 472 - 479
[30] Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells
Ou, Xiuyuan
Chen, Yan
Cheng, Xinxin
Zhang, Xumeng
He, Qiyang
ONCOLOGY REPORTS, 2014, 32 (06) : 2803 - 2809

← 1 2 3 4 5 →