Ratiometric Detection of Viscosity Using a Two-Photon Fluorescent Sensor

被引：117

作者：

Liu, Fei ^{[1
]}

Wu, Tong ^{[1
]}

Cao, Jianfang ^{[1
]}

Cui, Shuang ^{[2
]}

Yang, Zhigang ^{[1
]}

Qiang, Xinxin ^{[1
]}

Sun, Shiguo ^{[1
]}

Song, Fengling ^{[1
]}

Fan, Jiangli ^{[1
]}

Wang, Jingyun ^{[2
]}

Peng, Xiaojun ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2013年 / 19卷 / 05期

基金：

美国国家科学基金会;

关键词：

cells; fluorescence; sensors; two-photon microscopy; viscosity; MOLECULAR ROTORS; CELLS;

D O I：

10.1002/chem.201202646

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the thick of it: A carbazole-based cyanine derivative has been used as a ratiometric fluorescent sensor to detect mitochondrial viscosities over the range 1-950 cP within live cells and living tissues by using two-photon microscopy. Its success is based on the fast- and low-barrier rotation around its methine bond (see figure) and the huge increase of the fluorescence quantum yield (QY) from the loss of mobility in viscous media. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1548 / 1553

页数：6

共 50 条

[21] A ratiometric two-photon fluorescent probe for cysteine and homocysteine in living cells
Meng, Xiangming
Ye, Weipeng
Wang, Shuxin
Feng, Yan
Chen, Man
Zhu, Manzhou
Guo, Qingxiang
SENSORS AND ACTUATORS B-CHEMICAL, 2014, 201 : 520 - 525
[22] A ratiometric two-photon fluorescent probe for imaging hydrogen sulfide in lysosomes
Feng, Wenqi
Mao, Zhiqiang
Liu, Lingzhi
Liu, Zhihong
TALANTA, 2017, 167 : 134 - 142
[23] A cysteamine-selective two-photon fluorescent probe for ratiometric bioimaging
Sarkar, Avik R.
Heo, Cheol Ho
Kim, Eunjin
Lee, Hyo Won
Singh, Hardev
Kim, Jeong Jin
Kang, Hyuk
Kang, Chulhun
Kim, Hwan Myung
CHEMICAL COMMUNICATIONS, 2015, 51 (12) : 2407 - 2410
[24] A ratiometric two-photon fluorescent probe for the quantification of nitroreductase in hypoxic neurons
Tang, Zhiquan
Huang, Hong
Yao, Yuanyuan
Gao, Shumei
Lin, Bingyong
Zong, Qianshou
Hu, Wanpeng
Xu, Jianguo
Wang, Yangang
Guo, Longhua
CHEMICAL COMMUNICATIONS, 2025, 61 (03) : 560 - 563
[25] Two-Photon Absorbing Nanocrystal Sensors for Ratiometric Detection of Oxygen
McLaurin, Emily J.
Greytak, Andrew B.
Bawendi, Moungi G.
Nocera, Daniel G.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (36) : 12994 - 13001
[26] Ratiometric Detection of Mitochondrial Thiol with a Two-Photon Active AIEgen
Gu, Yuan
Zhao, Zheng
Niu, Guangle
Zhang, Ruoyao
Zhang, Han
Shan, Guo-Gang
Feng, Hai-Tao
Kwok, Ryan T. K.
Lam, Jacky W. Y.
Yu, Xiaoqiang
Tang, Ben Zhong
ACS APPLIED BIO MATERIALS, 2019, 2 (07) : 3120 - 3127
[27] Emission ratiometric imaging of intracellular zinc: Design of a benzoxazole fluorescent sensor and its application in two-photon microscopy
Taki, M
Wolford, JL
O'Halloran, TV
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (03) : 712 - 713
[28] Ratiometric Detection of γ-Glutamyltransferase in Human Colon Cancer Tissues Using a Two-Photon Probe
Kim, Yun Ji
Park, Sang Jun
Lim, Chang Su
Lee, Dong Jun
Noh, Choong-Kyun
Lee, Kiyoun
Shin, Sung Jae
Kim, Hwan Myung
ANALYTICAL CHEMISTRY, 2019, 91 (14) : 9246 - 9250
[29] Ultrafast supercontinuum sculpting for two-photon spectroscopy and microscopy of ratiometric fluorescent indicators
Chebotarev, Artem S.
Raevsky, Roman I.
Linovsky, Georgy N.
Kostyuk, Alexander I.
Belousov, Vsevolod V.
Fedotov, Andrei B.
Bilan, Dmitry S.
Lanin, Aleksandr A.
APPLIED PHYSICS LETTERS, 2024, 124 (24)
[30] Design of a two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorite in mitochondria
Li, Linke
Wang, Xinru
Sun, Chuan
Xu, Tingting
Yang, Zhijian
Zhang, Zhengsong
Feng, Yan
Fang, Min
Chen, Man
Wang, Xin
Meng, Xiangming
DYES AND PIGMENTS, 2020, 181

← 1 2 3 4 5 →