Ratiometric Detection of Viscosity Using a Two-Photon Fluorescent Sensor

被引：117

作者：

Liu, Fei ^{[1
]}

Wu, Tong ^{[1
]}

Cao, Jianfang ^{[1
]}

Cui, Shuang ^{[2
]}

Yang, Zhigang ^{[1
]}

Qiang, Xinxin ^{[1
]}

Sun, Shiguo ^{[1
]}

Song, Fengling ^{[1
]}

Fan, Jiangli ^{[1
]}

Wang, Jingyun ^{[2
]}

Peng, Xiaojun ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2013年 / 19卷 / 05期

基金：

美国国家科学基金会;

关键词：

cells; fluorescence; sensors; two-photon microscopy; viscosity; MOLECULAR ROTORS; CELLS;

D O I：

10.1002/chem.201202646

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the thick of it: A carbazole-based cyanine derivative has been used as a ratiometric fluorescent sensor to detect mitochondrial viscosities over the range 1-950 cP within live cells and living tissues by using two-photon microscopy. Its success is based on the fast- and low-barrier rotation around its methine bond (see figure) and the huge increase of the fluorescence quantum yield (QY) from the loss of mobility in viscous media. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1548 / 1553

页数：6

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