Evaluation of Virulence and Antimicrobial Resistance in Salmonella enterica Serovar Enteritidis Isolates from Humans and Chicken- and Egg-Associated Sources

被引:35
|
作者
Han, Jing [1 ]
Gokulan, Kuppan [1 ]
Barnette, Dustyn [1 ,2 ]
Khare, Sangeeta [1 ]
Rooney, Anthony W. [1 ,3 ]
Deck, Joanna [1 ]
Nayak, Rajesh [1 ]
Stefanova, Rossina [4 ]
Hart, Mark E. [1 ]
Foley, Steven L. [1 ]
机构
[1] US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA
[2] Ouachita Baptist Univ, Dept Biol, Arkadelphia, AR USA
[3] Univ Minnesota, Dept Chem, Morris, MN 56267 USA
[4] Arkansas Dept Hlth, Arkansas Publ Hlth Labs, Little Rock, AR 72205 USA
关键词
FIELD GEL-ELECTROPHORESIS; GENE PROFILES; GENOME; FOOD; DIVERSITY; INFECTION; POULTRY;
D O I
10.1089/fpd.2013.1518
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Salmonella enterica serovar Enteritidis is a leading cause of salmonellosis throughout the world and is most commonly associated with the consumption of contaminated poultry and egg products. Salmonella Enteritidis has enhanced ability to colonize and persist in extraintestinal sites within chickens. In this study, 54 Salmonella Enteritidis isolates from human patients (n=28), retail chicken (n=9), broiler farms (n=9), and egg production facilities (n=8) were characterized by antimicrobial susceptibility testing, plasmid analysis, genetic relatedness using XbaI and AvrII pulsed-field gel electrophoresis (PFGE), and the presence of putative virulence genes. Nine isolates were evaluated for their abilities to invade and survive in intestinal epithelial and macrophage cell lines. Overall, 56% (n=30) of isolates were resistant to at least one antimicrobial agent tested, yet no isolates showed resistance to more than three antimicrobials. All isolates carried a common approximate to 55-kb plasmid, with some strains containing additional plasmids ranging from 3 to 50kb. PFGE analysis revealed five XbaI and AvrII clusters. There were significant overlaps in the PFGE patterns of the isolates from human, chicken, and egg houses. All isolates tested PCR positive for iacP, purR, ttrB, spi4H, rmbA, sopE, invA, sopB, spvB, pagC, msgA, spaN, orgA, tolC, and sifA, and negative for iss, virB4, and sipB. Of the isolates selected for virulence testing, those containing the iron acquisition genes, iutA, sitA, and iucA, and approximate to 50-kb plasmids demonstrated among the highest levels of macrophage and epithelial cell invasion, which may indicate their importance in pathogenesis.
引用
收藏
页码:1008 / 1015
页数:8
相关论文
共 50 条
  • [21] RfaB, a galactosyltransferase, contributes to the resistance to detergent and the virulence of Salmonella enterica serovar Enteritidis
    Su, Jing
    Timbely, Dommo
    Zhu, Minmin
    Hua, Xiaomei
    Liu, Biao
    Pang, Yanjun
    Shen, Hengguan
    Qi, Jinliang
    Yang, Yonghua
    MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2009, 198 (03) : 185 - 194
  • [22] Antimicrobial resistance and molecular genotyping of Salmonella enterica serovar Enteritidis clinical isolates from Guizhou province of Southwestern China
    Wei, Xiaoyu
    You, Lv
    Wang, Dan
    Huang, He
    Li, Shijun
    Wang, Dingming
    PLOS ONE, 2019, 14 (09):
  • [23] Antimicrobial Resistance and Biofilm Formation Capacity of Salmonella enterica Serovar Enteritidis Strains Isolated from Poultry and Humans in Poland
    Cwiek, Katarzyna
    Korzekwa, Kamila
    Tabis, Aleksandra
    Bania, Jacek
    Bugla-Ploskonska, Gabriela
    Wieliczko, Alina
    PATHOGENS, 2020, 9 (08): : 1 - 22
  • [24] Molecular Characterization of Salmonella enterica Serotype Enteritidis Isolates from Humans by Antimicrobial Resistance, Virulence Genes, and Pulsed-Field Gel Electrophoresis
    Zou, Ming
    Keelara, Shivaramu
    Thakur, Siddhartha
    FOODBORNE PATHOGENS AND DISEASE, 2012, 9 (03) : 232 - 238
  • [25] Cecal CircRNAs Are Associated With the Response to Salmonella Enterica Serovar Enteritidis Inoculation in the Chicken
    Zheng, Linna
    Liu, Liying
    Lin, Lili
    Tang, Hui
    Fan, Xinzhong
    Lin, Hai
    Li, Xianyao
    FRONTIERS IN IMMUNOLOGY, 2019, 10
  • [26] Salmonella enterica Serovar Enteritidis Antimicrobial Peptide Resistance Genes Aid in Defense against Chicken Innate Immunity, Fecal Shedding, and Egg Deposition
    McKelvey, Jessica A.
    Yang, Ming
    Jiang, Yanhua
    Zhang, Shuping
    INFECTION AND IMMUNITY, 2014, 82 (12) : 5185 - 5202
  • [27] Antimicrobial Resistance, Resistance Genes and Virulence Genes in Salmonella Isolates From Chicken
    Liu, Yahong
    Yang, Yurong
    Liao, Xiaoping
    Li, Liang
    Lei, Chunyin
    Li, Lulu
    Sun, Jain
    Liu, Baotao
    JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2012, 11 (23): : 4423 - 4427
  • [28] Draft Genome Sequences of Salmonella enterica Serovar Enteritidis and Kentucky Isolates from Retail Poultry Sources
    Shi, Zhaohao
    Kaldhone, Pravin R.
    Khajanchi, Bijay K.
    Foley, Steven L.
    Ricke, Steven C.
    GENOME ANNOUNCEMENTS, 2018, 6 (14)
  • [29] Ceftiofur Resistance in Salmonella enterica Serovar Heidelberg from Chicken Meat and Humans, Canada
    Dutil, Lucie
    Irwin, Rebecca
    Finley, Rita
    Ng, Lai King
    Avery, Brent
    Boerlin, Patrick
    Bourgault, Anne-Marie
    Cole, Linda
    Daignault, Danielle
    Desruisseau, Andrea
    Demczuk, Walter
    Hoang, Linda
    Horsman, Greg B.
    Ismail, Johanne
    Jamieson, Frances
    Maki, Anne
    Pacagnella, Ana
    Pillai, Dylan R.
    EMERGING INFECTIOUS DISEASES, 2010, 16 (01) : 48 - 54
  • [30] Antimicrobial Resistance and Virulence Genes in Salmonella enterica Isolates from Dairy Cows
    Chuanchuen, Rungtip
    Ajariyakhajorn, Kittisak
    Koowatananukul, Chailai
    Wannaprasat, Wechsiri
    Khemtong, Sirintip
    Samngamnim, Sukuma
    FOODBORNE PATHOGENS AND DISEASE, 2010, 7 (01) : 63 - 69