Colon cancer cell chemosensitisation by fish oil emulsion involves apoptotic mitochondria pathway

被引:42
|
作者
Granci, Virginie [1 ]
Cai, Fang [1 ]
Lecumberri, Elena [1 ]
Clerc, Aurelie [1 ]
Dupertuis, Yves M. [1 ]
Pichard, Claude [1 ]
机构
[1] Univ Hosp Geneva, Unit Nutr, CH-1211 Geneva 14, Switzerland
基金
瑞士国家科学基金会;
关键词
Colorectal cancer; Fish oil; Oxaliplatin; 5-Fluorouracil; Irinotecan; Apoptosis; POLYUNSATURATED FATTY-ACIDS; NF-KAPPA-B; COLORECTAL-CANCER; DOCOSAHEXAENOIC ACID; NUDE-MICE; 5-FLUOROURACIL; CHEMOTHERAPY; EFFICACY; GROWTH; CARCINOMA;
D O I
10.1017/S000711451200308X
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Adjuvant use of safe compounds with anti-tumour properties has been proposed to improve cancer chemotherapy outcome. We aimed to investigate the effects of fish oil emulsion (FOE) rich in n-3 PUFA with the standard chemotherapeutic agents 5-fluorouracil (5-FU), oxaliplatin (OX) or irinotecan (IRI) on two human colorectal adenocarcinoma cells with different genetic backgrounds. The HT-29 (Bax(+/+)) and LS174T (Bax(-/-)) cells were co-treated for 24-72 h with 1 mu M-5-FU, 1 mu M-OX or 10 mu M-IRI and/or FOE dilution corresponding to 24 mu M-EPA and 20.5 mu M-DHA. Soyabean oil emulsion (SOE) was used as isoenergetic and isolipid control. Cell viability, apoptosis and nuclear morphological changes were evaluated by cytotoxic colorimetric assay, flow cytometry analysis with annexin V and 4',6'-diamidino-2-phenylindole staining, respectively. A cationic fluorescent probe was used to evaluate mitochondrial dysfunction, and protein expression involved in mitochondrial apoptosis was determined by Western blot. In contrast to SOE, co-treatment with FOE enhanced significantly the pro-apoptotic and cytotoxic effects of 5-FU, OX or IRI in HT-29 but not in LS174T cells (two-way ANOVA, P < 0.01). These results were confirmed by the formation of apoptotic bodies in HT-29 cells. A significant increase in mitochondrial membrane depolarisation was observed after the combination of 5-FU or IRI with FOE in HT-29 but not in LS174T cells (P < 0.05). Co-administration of FOE with the standard agents, 5-FU, OX and IRI, could be a good alternative to increase the efficacy of chemotherapeutic protocols through a Bax-dependent mitochondrial pathway.
引用
收藏
页码:1188 / 1195
页数:8
相关论文
共 50 条
  • [21] Evaluation of the Effects of the Flavonoid Apigenin on Apoptotic Pathway Gene Expression on the Colon Cancer Cell Line (HT29)
    Turktekin, Mehmet
    Konac, Ece
    Onen, H. Ilke
    Alp, Ebru
    Yilmaz, Akin
    Menevse, Sevda
    JOURNAL OF MEDICINAL FOOD, 2011, 14 (10) : 1107 - 1117
  • [22] Dietary fish oil and butyrate may protect against colon cancer by inducing mitochondria-dependent apoptosis in the promotion stage of carcinogenesis
    Newton, AH
    Turner, ND
    Hong, MY
    Davidson, LA
    Sanders, LM
    Chapkin, RS
    Murphy, ME
    Carroll, RJ
    Lupton, JR
    FASEB JOURNAL, 2004, 18 (04): : A10 - A10
  • [23] Chelerythrine-Induced Apoptotic Cell Death in HepG2 Cells Involves the Inhibition of Akt Pathway and the Activation of Oxidative Stress and Mitochondrial Apoptotic Pathway
    Lin, Yanling
    Zhang, Qinzhi
    Xie, Baofu
    Jiang, Haiyang
    Shen, Jianzhong
    Tang, Shusheng
    Dai, Chongshan
    ANTIOXIDANTS, 2022, 11 (09)
  • [24] Emodin inhibits colon cancer by modulating apoptotic and cell survival signals
    Saunders, Ian T.
    Kapur, Neeraj
    Mir, Hina
    Singh, Shailesh
    CANCER RESEARCH, 2018, 78 (13)
  • [25] Induction of mitochondria! apoptotic pathway by raloxifene and estrogen in human endometrial stromal ThESC cell line
    Nikolic, Ivana
    Andjelkovic, Marija
    Zaric, Milan
    Zelen, Ivanka
    Canovic, Petar
    Milosavljevic, Zoran
    Mitrovic, Marina
    ARCHIVES OF MEDICAL SCIENCE, 2017, 13 (02) : 293 - 301
  • [26] Effects of a fish oil-based emulsion on rat hepatoma cell invasion in culture
    Hagi, Akifumi
    Nakayama, Mitsuo
    Miura, Yutaka
    Yagasaki, Kazumi
    NUTRITION, 2007, 23 (11-12) : 871 - 877
  • [27] Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line
    Cincin, Zeynep Birsu
    Unlu, Miray
    Kiran, Bayram
    Bireller, Elif Sinem
    Baran, Yusuf
    Cakmakoglu, Bedia
    PATHOLOGY & ONCOLOGY RESEARCH, 2015, 21 (02) : 333 - 338
  • [28] Ras Signaling Pathway in the Chemopreventive Action of Different Ratios of Fish Oil and Corn Oil in Experimentally Induced Colon Carcinogenesis
    Kansal, S.
    Negi, A. K.
    Bhatnagar, A.
    Agnihotri, N.
    NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2012, 64 (04): : 559 - 568
  • [29] Potentiation of human colon cancer cell apoptotic cell death by inhibition of ceramidase/glucosylceramide synthase
    Dionne, S
    Levesque, D
    Seidman, EG
    GASTROENTEROLOGY, 2003, 124 (04) : A598 - A598