FurA influences heterocyst differentiation in Anabaena sp PCC 7120

被引：16

作者：

Gonzalez, Andres ^{[1
,2
]}

Valladares, Ana ^{[3
]}

Luisa Peleato, M. ^{[1
,2
]}

Fillat, Maria F. ^{[1
,2
]}

机构：

[1] Univ Zaragoza, Dept Bioquim & Biol Mol & Celular, E-50009 Zaragoza, Spain

[2] Univ Zaragoza, Inst Biocomput & Fis Sistemas Complejos BIFI, E-50009 Zaragoza, Spain

[3] Univ Seville, CSIC, Inst Bioquim Vegetal & Fotosintesis, Ctr Invest Cient Isla Cartuja, E-41092 Seville, Spain

来源：

FEBS LETTERS | 2013年 / 587卷 / 16期

关键词：

Heterocyst differentiation; Ferric uptake regulator; Anabaena; SP STRAIN PCC-7120; NTCA TRANSCRIPTION FACTOR; MARINE NITROGEN-FIXATION; ENVELOPE POLYSACCHARIDE; PATTERN-FORMATION; IRON LIMITATION; CYANOBACTERIUM; EXPRESSION; GENES; PROTEIN;

D O I：

10.1016/j.febslet.2013.07.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Anabaena sp. PCC 7120, FurA is a global transcriptional regulator whose expression is strongly induced by NtcA in proheterocysts and remains stably expressed in mature heterocysts. In the present study, overexpression of furA partially suppressed heterocyst differentiation by impairing morphogenesis at an early stage. Recombinant purified FurA specifically bound in vitro to the promoter regions of ntcA, while quantitative RT-PCR analyses indicated that furA overexpression strongly affected the transient increase of ntcA expression that occurs shortly after nitrogen step-down. Overall, the results suggest a connection between iron homeostasis and heterocyst differentiation via FurA, by modulating the expression of ntcA. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2682 / 2690

页数：9

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