Lack of Correlation between HERV-K Expression and HIV-1 Viral Load in Plasma Specimens

被引：0
|
作者：
Esqueda, Daniel [1 ]
Xu, Fangling [2 ]
Moore, Yuliana [1 ]
Yang, Zhen [2 ]
Huang, Gengming [2 ]
Lennon, Patrick A. [1 ]
Hu, Peter C. [1 ]
Dong, Jianli [2 ]
机构：
[1] Univ Texas MD Anderson Canc Ctr, Sch Hlth Sci, Mol Genet Technol Program, Houston, TX 77030 USA
[2] Univ Texas Med Branch, Mol Diagnost Lab, Galveston, TX 77555 USA
来源：
ANNALS OF CLINICAL AND LABORATORY SCIENCE | 2013年 / 43卷 / 02期
关键词：
HERV-K expression; HIV-1 viral load; DNase I digestion; HUMAN ENDOGENOUS RETROVIRUSES; T-CELL RESPONSES; RNA TITERS; MELANOMA;
D O I：
暂无
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
HERV-K viral RNA has been reported in plasma specimens of HIV-1 infected individuals. Emerging data support the regulation and functional interaction between HERV-K and HIV-1, which warrant development of accurate HERV-K assays to evaluate HERV-K activation. In this study, we examined HERV-K RNA expression after careful removal of "contaminating" cellular DNA using DNase I. We found that DNase I digestion effectively reduced HERV-K RT-PCR positive signal. We also found that levels of HERV-K expression did not correlate with HIV-1 viral load. Our study is in agreement with the published studies on HERV-K activation in HIV-1 positive plasma specimens, and in addition, calls for careful removal of cellular DNA to accurately evaluate HERV-K RNA expression.
引用
收藏
页码：122 / 125
页数：4
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