Lack of Correlation between HERV-K Expression and HIV-1 Viral Load in Plasma Specimens

被引：0

作者：

Esqueda, Daniel ^{[1
]}

Xu, Fangling ^{[2
]}

Moore, Yuliana ^{[1
]}

Yang, Zhen ^{[2
]}

Huang, Gengming ^{[2
]}

Lennon, Patrick A. ^{[1
]}

Hu, Peter C. ^{[1
]}

Dong, Jianli ^{[2
]}

机构：

[1] Univ Texas MD Anderson Canc Ctr, Sch Hlth Sci, Mol Genet Technol Program, Houston, TX 77030 USA

[2] Univ Texas Med Branch, Mol Diagnost Lab, Galveston, TX 77555 USA

来源：

ANNALS OF CLINICAL AND LABORATORY SCIENCE | 2013年 / 43卷 / 02期

关键词：

HERV-K expression; HIV-1 viral load; DNase I digestion; HUMAN ENDOGENOUS RETROVIRUSES; T-CELL RESPONSES; RNA TITERS; MELANOMA;

D O I：

暂无

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

HERV-K viral RNA has been reported in plasma specimens of HIV-1 infected individuals. Emerging data support the regulation and functional interaction between HERV-K and HIV-1, which warrant development of accurate HERV-K assays to evaluate HERV-K activation. In this study, we examined HERV-K RNA expression after careful removal of "contaminating" cellular DNA using DNase I. We found that DNase I digestion effectively reduced HERV-K RT-PCR positive signal. We also found that levels of HERV-K expression did not correlate with HIV-1 viral load. Our study is in agreement with the published studies on HERV-K activation in HIV-1 positive plasma specimens, and in addition, calls for careful removal of cellular DNA to accurately evaluate HERV-K RNA expression.

引用

页码：122 / 125

页数：4

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