Genetic characterization of local goats of Karnataka by microsatellite marker analysis

被引：4
|
作者：
Jayashree, R. [1 ]
Jayashankar, M. R. [1 ]
Nagaraja, C. S. [1 ]
Shrikrishna, Isloor [1 ]
Satyanarayana, K. [1 ]
机构：
[1] Vet Coll, Dept Anim Genet & Breeding, Bangalore 560024, Karnataka, India
来源：
INDIAN JOURNAL OF ANIMAL RESEARCH | 2019年 / 53卷 / 01期
关键词：
Local goats; Microsatellite; BREED; VARIABILITY;
D O I：
10.18805/ijar.B-3458
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The diversity status of local goats of Karnataka was studied by using microsatellite marker analysis. The genomic DNA from unrelated local goats were PCR-amplified with a panel of 23 microsatellite markers. Microsatellite PCR products were multiplexed and run on capillary based genetic analyser and the raw data obtained was analysed. Totally 158 alleles were observed and the number of alleles ranged from three (ILSTS005 and OarJMP29) to 13 (RM088). The number of effective alleles ranged from 2.25 (ILSTS005) to 8.40 (RM088) in all the 23 loci studied. The mean observed heterozygosity (Ho) was 0.4698 +/- 0.2214 [range 0 (ETH225) to 0.8462 (ILSTS034)] and the mean expected heterozygosity (He) was 0.7471 +/- 0.1098 [range 0.5656 (ILSTS005) to 0.9138 (SRCRSP 8)] indicating the heterogenous nature of the local goat population of Karnataka.
引用
收藏
页码：19 / 23
页数：5
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