Label-free, disposable fiber-optic biosensors for DNA hybridization detection

被引：35
|
作者：
Yin, Ming-Jie [1 ]
Wu, Chuang [1 ]
Shao, Li-yang [1 ]
Chan, Wing Kin Edward [1 ]
Zhang, A. Ping [1 ]
Lu, Chao [2 ]
Tam, Hwa-yaw [1 ]
机构：
[1] Hong Kong Polytech Univ, Photon Res Ctr, Dept Elect Engn, Kowloon, Hong Kong, Peoples R China
[2] Hong Kong Polytech Univ, Photon Res Ctr, Dept Elect & Informat Engn, Kowloon, Hong Kong, Peoples R China
来源：
ANALYST | 2013年 / 138卷 / 07期
关键词：
SURFACE-PLASMON RESONANCE; BY-LAYER ADSORPTION; MULTILAYER FILMS; POLYELECTROLYTE MULTILAYERS; MODAL INTERFEROMETER; GOLD SURFACES; THIN-FILMS; SENSOR; NANOPARTICLES; PERFORMANCE;
D O I：
10.1039/c3an36791f
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)(4), (PEI/PAA)(4.5) and (PEI/PAA)(4)(PEI/DNA)(1) multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 mu M. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 mM and can even distinguish the number of matched bases of ssDNA chains.
引用
收藏
页码：1988 / 1994
页数：7
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