Development of a multiplexing potency assay using upconverting nanoparticles-based luminescence resonance energy transfer

A homogeneous particle-based immunoassay using upconverting nanoparticles (UCNPs) has been developed for multiplexing potency analysis of two different therapeutic monoclonal antibodies (mAbs) in a fixed-combination formulation.The UCNP, considered as the best donor lumiphore for luminescence resonance energy transfer (LRET), offers long lasting excitation state and increased signal-to-noise (S/N) ratio due to low autofluorescence effect and light scattering from near infrared (NIR) excitation. In this study, the dose-response curves for each therapeutic mAb were generated using two distinct UCNPs. This proof-of-concept LRET-based immunoassay demonstrated a novel approach for increasing testing throughput and analyzing the potency of mixed therapeutic mAbs in co-formulated products.