Effect of different nuclear localization sequences on the immune responses induced by a MIDGE vector encoding bovine herpesvirus-1 glycoprotein D

被引：23
|
作者：
Zheng, Chunfu
Juhls, Christiane
Oswald, Detlef
Sack, Florian
Westfehling, Ines
Wittig, Burghardt
Babiuk, Lorne A.
van Drunen Littel-van den Hurk, Sylvia
机构：
[1] Univ Saskatchewan, Vaccine & Infect Dis Org, Saskatoon, SK S7N 5E3, Canada
[2] Mologen AG, D-14195 Berlin, Germany
[3] Free Univ Berlin, Inst Mol Biol & Biochem, D-14195 Berlin, Germany
来源：
VACCINE | 2006年 / 24卷 / 21期
基金：
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词：
MIDGE; NLS; tgD;
D O I：
10.1016/j.vaccine.2005.08.046
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
To optimize the efficiency of a Minimalistic Immunogenically Defined Gene Expression (MIDGE) vector, peptides containing proven (SV40 T-antigen and bovine herpesvirus-1 VP8) or putative (herpes simplex virus-1 VP22) nuclear localization signals (NLSs) were linked to a MIDGE (R) vector encoding a truncated, secreted form of BHV-1 glycoprotein D (tgD) (MIDGE-tgD). Conjugation of an NLS to the MIDGE-tgD vector improved the tgD expression in vitro and the humoral and cellular immune responses induced in mice in vivo. The NLS from BHV-1 VP8 was most efficient at enhancing the tgD production and tgD-specific immune responses when conjugated to the MIDGE-tgD vector. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4625 / 4629
页数：5
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