The non-mimetic Afrotropical spider genus Cambalida Simon, 1909, placed within a subfamily of pre-dominantly ant-mimicking spiders (Araneae: Corinnidae: Castianeirinae), is revised. Three species are transferred from Castianeira Keyserling, 1879 to Cambalida: C. deminuta (Simon, 1909), comb. n., C. fulvipes (Simon, 1896), comb. n. and C. loricifera (Simon, 1885), comb. n., A fourth species, C. fagei (Caporiacco, 1939), comb. n., is transferred from Brachyphaea Simon, 1895 to Cambalida. Two species. Castianeira depygata Strand, 1916, syn. n. and C. mestrali Lessert, 1921, syn. n., are considered junior synonyms of C. fulvipes. The males of C. deminuta and C. loricifera are redescribed and their unknown females are described for the first time. The female and male of C. fulvipes and C. coriacea Simon, 1909 are also redescribed. The type material of the type species of the genus, C. insulana Simon, 1909 from Pagalu (Annobon) Island, is lost, and only immature specimens have been subsequently collected from a nearby island. The species is regarded as a nomen dubium until fresh adult material can be collected. A replacement name, Cambalida simoni nom. n. is proposed for Cambalida fulvipes Simon, 1909, the latter being a secondary junior homonym of Cambalida fulvipes (Simon, 1896). The type material of this species is also lost and it is too considered nomen dubium. The following new species are described: C. compressa sp., n. from West Africa, C. dippenaarae sp. n. from southern Africa, C. griswoldi sp. n. and C. lineata sp. n., from Madagascar, and C. unica sp. n. from Cameroon. Notes are provided on the biology of each species and the distribution of the genus in the Afrotropical Region.