genodiveversion 3.0 is a user-friendly program for the analysis of population genetic data. This version presents a major update from the previous version and now offers a wide spectrum of different types of analyses.genodivehas an intuitive graphical user interface that allows direct manipulation of the data through transformation, imputation of missing data, and exclusion and inclusion of individuals, population and/or loci. Furthermore,genodiveseamlessly supports 15 different file formats for importing or exporting data from or to other programs. One major feature ofgenodiveis that it supports both diploid and polyploid data, up to octaploidy (2n = 8x) for some analyses, but up to hexadecaploidy (2n = 16x) for other analyses. The different types of analyses offered bygenodiveinclude multiple statistics for estimating population differentiation (phi(ST),F-ST,FMODIFIER LETTER PRIMEST,G(ST),GMODIFIER LETTER PRIMEST,GMODIFIER LETTER PRIMEMODIFIER LETTER PRIMEST,D-est,R-ST,rho), analysis of molecular variance-basedK-means clustering, Hardy-Weinberg equilibrium, hybrid index, population assignment, clone assignment, Mantel test, Spatial Autocorrelation, 23 ways of calculating genetic distances, and both principal components and principal coordinates analyses. A unique feature ofgenodiveis that it can also open data sets with nongenetic variables, for example environmental data or geographical coordinates that can be included in the analysis. In addition,genodivemakes it possible to run several external programs (lfmm,structure,instructandvegan) directly from its own user interface, avoiding the need for data reformatting and use of the command line.genodiveis available for computers running Mac OS X 10.7 or higher and can be downloaded freely from: .