High production of β-glucosidase from a marine Aspergillus niger immobilized on towel gourd vegetable sponges

To produce p-glucosidase by consecutive batch fermentation, a marine Aspergillus niger was immobilized on a natural carrier, towel gourd vegetable sponges. The immobilized mycelia were 0.15 g/g carrier with the immobilized biomass percentage of over 95%. The immobilized mycelia possessed the long durability (22.5 days). The maximum production occurred 1.5 day earlier by the immobilized mycelia than by the free mycelia. beta-Glucosidase production of five consecutive batches was over 110 U/mL. At high salinity, the activity and stability of beta-glucosidase from the marine A. niger increased remarkable. Immobilizing the marine A. niger on the novel natural carrier achieved the efficient production of beta-glucosidase. (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.