Quercetin Protects Hepatocytes from Apoptosis Induced by H2O2

In our present study, we firstly evaluated the effects of quercetin to hepatocytes apoptosis and cell viability in different concentrations and times. Depending on these results, we determined the optimum time and concentration. Secondly, the cells were divided into 4 groups: NC, model, treated by quercetina, and Acte+LY294002 (AKT inhibitor) groups. The cell apoptosis rates of difference groups were measured by flow cytometry. Western blot was used to investigate protein levels of Cyt-C(cyt), CytC-(Mit), AKT, GSK-3 beta, Bax and Bcl-xl. Compared with model group, acteoside can increase the cell viability and decrease the cell apoptosis rate. Meanwhile, the Cyt-C(cyt), Cyt-C(Mit), AKT, GSK-3 beta, Bax and Bcl-xl protein expressions of quercetin treated group were significantly differences compared with model group (P < 0.05, respectively). These results suggested that quercetin had effects to protected fetal hepatocytes of mice from H2O2-induced apoptosis by PI3K/AKT/GSK3 beta signal pathway.