Interrelation between cerebral energy metabolism and behaviour in a rat model of permanent brain vessel occlusion

被引:45
|
作者
Plaschke, K
Yun, SW
Martin, E
Hoyer, S
Bardenheuer, HJ
机构
[1] Univ Heidelberg, Anesthesiol Clin, D-69120 Heidelberg, Germany
[2] Univ Heidelberg, Dept Pathochem & Gen Neurochem, D-69120 Heidelberg, Germany
关键词
four-vessel occlusion; energy metabolism; adenosine; reference memory; working memory; passive avoidance;
D O I
10.1016/S0006-8993(99)01427-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The present study investigates the interrelation between cerebral energy metabolism and memory capacities after acute and permanent occlusions of carotid and vertebral arteries in adult Wistar rats (n = 60). Tissue ATP, phosphocreatine, ADP, AMP and adenosine concentrations were determined in rat brain by high-pressure liquid chromatography (HPLC) analysis. Lactate and pyruvate were measured spectrophotometrically. Rats underwent psychometric testing by means of a holeboard test, closed field activity, and passive avoidance behaviour. Acute cerebral ischaemia was associated with a substantial deficit in energy load (- 50%). Cortical adenosine and lactate exhibited a 7- and a 10-fold increase, respectively, in concentration. After 2 weeks of four-vessel occlusion, cortical ATP and phosphocreatine showed a partial enhancement in their concentrations if compared with acute ischaemia. Consequently, energy load (mu mol/g) increased from 0.59 to 1.42 in cerebral cortex and from 0.58 to 1.14 in hippocampus under conditions of acute and permanent ischaemia, respectively. While lactate was normalized, adenosine showed a 2-fold increase in its cortical concentration. All animals improved their abilities in learning, memory and cognition after a 7-day training period. Acute vessel occlusion severely decreased working memory (WM), reference memory (RM) and locomotor activity. Simultaneously, the passive avoidance test showed a significant reduction in latency time from 247 +/- 85 s (sham) to 145 +/- 132 s. The partial improvement in brain energy state was accompanied by a relative improvement in WM and RM, although both memory capacities remained significantly lower than in controls. The data of the present study demonstrate a Linear relationship between cerebral energy metabolism and brain memory capacities after acute and permanent vessel occlusions in rats. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:320 / 329
页数:10
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