Whole plant regeneration from the shootapical meristem of Opuntia ficus-indica Mill. (Cactaceae)

被引:0
|
作者
Llamoca-Zárate, RM
Aguiar, LF
Landsmann, J
Campos, FAP
机构
[1] Univ Fed Ceara, Dept Biochem & Mol Biol, BR-60001970 Fortaleza, Ceara, Brazil
[2] Fed Biol Res Ctr Agr & Forestry, Inst Plant Virol Microbiol & Biosafety, D-38104 Braunschweig, Germany
来源
JOURNAL OF APPLIED BOTANY-ANGEWANDTE BOTANIK | 1999年 / 73卷 / 3-4期
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D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Lack of reliable and quick protocols for the in vitro culture of the prickly-pear has proven to be one of the hindrances in the application of transformation protocols to this crop. Here we present a protocol for the initiation of shoot apex elongation and rooting of these shoots. Shoot apices were isolated from germinated seedlings and placed on MURASHIGE and SKOOG (MS) medium containing 6-benzylaminopurine (BA) and gibberellic acid (GA). When shoots became visible, the explants were transferred to MS-medium containing BA and indole-3-acetic acid (IAA). Once shoot elongation occurred, they were transferred to MS-medium containing only one plant growth regulator, IAA. Within three weeks, the shoots began to root and could be transferred to soil. At the same time, this protocol outlines a method for the mass propagation of the prickly-pear based on the proliferation of apical buds obtained from shoots in the elongation phase.
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页码:83 / 85
页数:3
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