Whole plant regeneration from the shootapical meristem of Opuntia ficus-indica Mill. (Cactaceae)

被引：0

作者：

Llamoca-Zárate, RM

Aguiar, LF

Landsmann, J

Campos, FAP

机构：

[1] Univ Fed Ceara, Dept Biochem & Mol Biol, BR-60001970 Fortaleza, Ceara, Brazil

[2] Fed Biol Res Ctr Agr & Forestry, Inst Plant Virol Microbiol & Biosafety, D-38104 Braunschweig, Germany

来源：

JOURNAL OF APPLIED BOTANY-ANGEWANDTE BOTANIK | 1999年 / 73卷 / 3-4期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Lack of reliable and quick protocols for the in vitro culture of the prickly-pear has proven to be one of the hindrances in the application of transformation protocols to this crop. Here we present a protocol for the initiation of shoot apex elongation and rooting of these shoots. Shoot apices were isolated from germinated seedlings and placed on MURASHIGE and SKOOG (MS) medium containing 6-benzylaminopurine (BA) and gibberellic acid (GA). When shoots became visible, the explants were transferred to MS-medium containing BA and indole-3-acetic acid (IAA). Once shoot elongation occurred, they were transferred to MS-medium containing only one plant growth regulator, IAA. Within three weeks, the shoots began to root and could be transferred to soil. At the same time, this protocol outlines a method for the mass propagation of the prickly-pear based on the proliferation of apical buds obtained from shoots in the elongation phase.

引用

页码：83 / 85

页数：3

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