Characterization of EST-derived microsatellites for gene mapping and evolutionary genomics in turbot

被引：31
|
作者：
Bouza, C. [1 ]
Hermida, M. [1 ]
Millan, A. [1 ]
Vilas, R. [1 ]
Vera, M. [1 ]
Fernandez, C. [1 ]
Calaza, M. [2 ]
Pardo, B. G. [1 ]
Martinez, P. [1 ]
机构：
[1] Univ Santiago de Compostela, Fac Vet, Dept Xenet, Lugo 27002, Spain
[2] Univ Santiago de Compostela, Dept Matemat Aplicada, Santiago De Compostela 15782, Spain
来源：
ANIMAL GENETICS | 2008年 / 39卷 / 06期
关键词：
expressed sequence tags mapping; microsatellites; Scophthalmus maximus; turbot; type I markers;
D O I：
10.1111/j.1365-2052.2008.01784.x
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The detection of microsatellite sequences within expressed sequence tags (ESTs) connects potential markers with specific genes, generating type I markers. We have developed and mapped by linkage analysis a set of EST-derived microsatellites in the turbot, Scophthalmus maximus. One hundred and ninety-one microsatellites were identified from 9256 turbot ESTs. Primer design was possible with 98 microsatellites. After genotyping 25 wild turbot and the parents of two reference families for linkage analysis, 43 EST-derived microsatellites were selected because they met technical and polymorphism criteria. A final set of 31 EST-derived microsatellites could be mapped to 17 linkage groups of the turbot consensus map based on 242 anonymous microsatellites. Twenty-four microsatellite-containing ESTs were functionally annotated, confirming them as type I markers. Nineteen were mapped in the turbot consensus map. These EST-derived microsatellites constitute useful tools for genome scanning of turbot populations, marker-assisted selection programmes and comparative mapping.
引用
收藏
页码：666 / 670
页数：5
相关论文
共 50 条
  • [21] The use of EST-derived microsatellites as markers in the development of a genetic map in kiwifruit
    Fraser, L. G.
    McNeilage, M. A.
    Tsang, G. K.
    De Silva, H. N.
    MacRae, E. A.
    PROCEEDINGS OF THE 6TH INTERNATIONAL SYMPOSIUM ON KIWIFRUIT, VOLS 1 AND 2, 2007, (753): : 169 - 175
  • [22] Characterization, development and exploitation of EST-derived microsatellites in Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)
    Chao-Tian Xie
    Chang-Sheng Chen
    De-Hua Ji
    Yan Xu
    Journal of Applied Phycology, 2009, 21 : 367 - 374
  • [23] Characterization, development and exploitation of EST-derived microsatellites in Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)
    Xie, Chao-Tian
    Chen, Chang-Sheng
    Ji, De-Hua
    Xu, Yan
    JOURNAL OF APPLIED PHYCOLOGY, 2009, 21 (03) : 367 - 374
  • [24] Genomic distribution and characterization of EST-derived resistance gene analogs (RGAs) in sugarcane
    Rossi, M
    Araujo, PG
    Paulet, F
    Garsmeur, O
    Dias, VM
    Chen, H
    Van Sluys, MA
    D'Hont, A
    MOLECULAR GENETICS AND GENOMICS, 2003, 269 (03) : 406 - 419
  • [25] Genomic distribution and characterization of EST-derived resistance gene analogs (RGAs) in sugarcane
    M. Rossi
    P. G. Araujo
    F. Paulet
    O. Garsmeur
    V. M. Dias
    H. Chen
    M.-A. Van Sluys
    A. D'Hont
    Molecular Genetics and Genomics, 2003, 269 : 406 - 419
  • [26] Identification, characterization and mapping of EST-derived SSRs from the cacao–Ceratocystis cacaofunesta interaction
    Rogério Mercês Ferreira Santos
    Didier Clement
    Lívia Santos Lima Lemos
    Thierry Legravre
    Claire Lanaud
    Raymond J. Schnell
    Jose Luis Pires
    Uilson Vanderlei Lopes
    Fabienne Micheli
    Karina Peres Gramacho
    Tree Genetics & Genomes, 2013, 9 : 117 - 127
  • [27] A Selected Set of EST-Derived Microsatellites, Polymorphic and Transferable across 6 Species of Eucalyptus
    Faria, Danielle A.
    Celia Mamani, Eva Maria
    Pappas, Marilia R.
    Pappas, Georgios Joannis, Jr.
    Grattapaglia, Dario
    JOURNAL OF HEREDITY, 2010, 101 (04) : 512 - 520
  • [28] Mining, genetic mapping and expression analysis of EST-derived resistance gene homologs (RGHs) in cotton
    Gaofeng Ren
    Ximei Li
    Zhongxu Lin
    BMC Plant Biology, 14
  • [29] Mining, genetic mapping and expression analysis of EST-derived resistance gene homologs (RGHs) in cotton
    Ren, Gaofeng
    Li, Ximei
    Lin, Zhongxu
    BMC PLANT BIOLOGY, 2014, 14
  • [30] Genetic mapping of new cotton fiber loci using EST-derived microsatellites in an interspecific recombinant inbred line cotton population
    Park, YH
    Alabady, MS
    Ulloa, M
    Sickler, B
    Wilkins, TA
    Yu, J
    Stelly, DM
    Kohel, RJ
    El-Shihy, OM
    Cantrell, RG
    MOLECULAR GENETICS AND GENOMICS, 2005, 274 (04) : 428 - 441
12345