Visualization of Aspergillus fumigatus biofilms with Scanning Electron Microscopy and Variable Pressure-Scanning Electron Microscopy: A comparison of processing techniques

被引：19

作者：

Joubert, Lydia-Marie ^{[1
]}

Ferreira, Jose A. G. ^{[2
,3
,5
]}

Stevens, David A. ^{[2
,3
]}

Nazik, Hasan ^{[2
,3
,6
]}

Cegelski, Lynette ^{[4
]}

机构：

[1] Stanford Univ, Sch Med, Cell Sci Imaging Facil, Stanford, CA 94305 USA

[2] Stanford Univ, Div Infect Dis & Geog Med, Stanford, CA 94305 USA

[3] Calif Inst Med Res, San Jose, CA 95128 USA

[4] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[5] Fac Saude & Ecol Humana FASEH, Sch Med, Vespasiano, Brazil

[6] Istanbul Univ, Dept Med Microbiol, Istanbul, Turkey

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 132卷

基金：

美国国家科学基金会;

关键词：

Aspergillus fumigatus; Biofilms; Scanning Electron Microscopy; Variable Pressure-SEM; Processing techniques; STREPTOCOCCUS-MUTANS BIOFILM; EXTRACELLULAR-MATRIX; RUTHENIUM RED; COLONIZATION; TETROXIDE; FIXATIVES;

D O I：

10.1016/j.mimet.2016.11.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aspergillus fumigatus biofilms consist of a three-dimensional network of cellular hyphae and extracellular matrix. They are involved in infections of immune-compromised individuals, particularly those with cystic fibrosis. These structures are associated with persistence of infection, resistance to host immunity, and antimicrobial resistance. Thorough understanding of structure and function is imperative in the design of therapeutic drugs. Optimization of processing parameters, including aldehyde fixation, heavy metal contrasting, drying techniques and Ionic Liquid treatment, was undertaken for an ultrastructural approach to understand cellular and extracellular biofilm components. Conventional and Variable Pressure Scanning Electron Microscopy were applied to analyze the structure of biofilms attached to plastic and formed at an air-liquid interface. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：46 / 55

页数：10

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