Utilizing nanopore sequencing technology for the rapid and comprehensive characterization of eleven HLA loci; addressing the need for deceased donor expedited HLA typing

被引：41

作者：

Mosbruger, Timothy L. ^{[1
]}

Dinou, Amalia ^{[1
]}

Duke, Jamie L. ^{[1
]}

Ferriola, Deborah ^{[1
]}

Mehler, Hilary ^{[1
]}

Pagkrati, Ioanna ^{[1
]}

Damianos, Georgios ^{[1
]}

Mbunwe, Eric ^{[2
,4
]}

Sarmady, Mahdi ^{[1
,3
]}

Lyratzakis, Ioannis ^{[1
]}

Tishkoff, Sarah A. ^{[2
]}

Dinh, Anh ^{[1
,3
]}

Monos, Dimitri S. ^{[1
,3
]}

机构：

[1] Childrens Hosp Philadelphia, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA

[2] Univ Penn, Perelman Sch Med, Dept Genet, Philadelphia, PA 19104 USA

[3] Univ Penn, Perelman Sch Med, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA

[4] Univ Massachusetts Lowell, Dept Chem, Lowell, MA USA

来源：

HUMAN IMMUNOLOGY | 2020年 / 81卷 / 08期

关键词：

Human Leukocyte Antigens; NGS; Oxford Nanopore sequencing; Transplantation; HLA genotyping; HIGH-RESOLUTION; MISMATCH;

D O I：

10.1016/j.humimm.2020.06.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The comprehensive characterization of human leukocyte antigen (HLA) genomic sequences remains a challenging problem. Despite the significant advantages of next-generation sequencing (NGS) in the field of Immunogenetics, there has yet to be a single solution for unambiguous, accurate, simple, cost-effective, and timely genotyping necessary for all clinical applications. This report demonstrates the benefits of nanopore sequencing introduced by Oxford Nanopore Technologies (ONT) for HLA genotyping. Samples (n = 120) previously characterized at high-resolution three-field (HR-3F) for 11 loci were assessed using ONT sequencing paired to a single-plex PCR protocol (Holotype) and to two multiplex protocols OmniType (Omixon) and NGSgo (R)-MX6-1 (GenDx). The results demonstrate the potential of nanopore sequencing for delivering accurate HR-3F typing with a simple, rapid, and cost-effective protocol. The protocol is applicable to time-sensitive applications, such as deceased donor typings, enabling better assessments of compatibility and epitope analysis. The technology also allows significantly shorter turnaround time for multiple samples at a lower cost. Overall, the nanopore technology appears to offer a significant advancement over current next-generation sequencing platforms as a single solution for all HLA genotyping needs.

引用

页码：413 / 422

页数：10

共 21 条

[21] AUTOMATION MEDIATED LARGE-SCALE HLA-DR-BETA- AND DQ-BETA-OLIGONUCLEOTIDE TYPING OF AN UNRELATED MARROW DONOR REGISTRY, THE RAPID MOLECULAR CROSS-MATCH BASED SELECTION OF MATCHING DONOR CANDIDATES AND CONSECUTIVE PROCUREMENT OF CLASS-II DR-BETA, DQ-BETA AND DP-BETA IDENTICAL DONOR-RECIPIENT PAIRS BY DIRECT AUTOMATIC HLA SEQUENCING
WERNET, P
ENCZMANN, J
UHRBERG, M
KNIPPER, A
ASSHEURER, B
KOGLER, G
KIESEL, U
EXPERIMENTAL HEMATOLOGY, 1993, 21 (08) : 1111 - 1111

← 1 2 3 →