Statistical optimization of activity and stability of β-xylanase produced by newly isolated Thermomyces lanuginosus THKU-49 using central composite design

被引：0
|
作者：
Khucharoenphaisan, Khwanchai [1 ]
Tokuyama, Shinji [2 ]
Kitpreechavanich, Vichien [1 ]
机构：
[1] Kasetsart Univ, Fac Sci, Dept Microbiol, Bangkok 10900, Thailand
[2] Shizuoka Univ, Fac Agr, Dept Appl Biol Chem, Shizuoka 4228529, Japan
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2008年 / 7卷 / 20期
关键词：
-xylanase; optimization; central composite design;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A factorial design was performed to find the best conditions of pH and temperature for beta-xylanase activity and to maintain its activity for prolonged periods of time of pure xylanase produced by newly isolated Thermomyces lanuginosus THKU-49. The central composite design (CCD) used for the analysis of treatment combinations showed that a regression models of optimization of xylanase activity and xylanase stability were good agreement to experimental results with R-2 = 0.98 and 0.99, respectively. The maximum activity of xylanase was obtained at 66 degrees C and pH 6.3. The temperature and pH for maximum enzyme stability was 70 degrees C and pH 7.3, respectively. Under this condition xylanase having half-life of 825 min indicated the highest thermostable xylanase.
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页码：3599 / 3602
页数：4
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