Statistical optimization of activity and stability of β-xylanase produced by newly isolated Thermomyces lanuginosus THKU-49 using central composite design

被引：0
|
作者：
Khucharoenphaisan, Khwanchai [1 ]
Tokuyama, Shinji [2 ]
Kitpreechavanich, Vichien [1 ]
机构：
[1] Kasetsart Univ, Fac Sci, Dept Microbiol, Bangkok 10900, Thailand
[2] Shizuoka Univ, Fac Agr, Dept Appl Biol Chem, Shizuoka 4228529, Japan
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2008年 / 7卷 / 20期
关键词：
-xylanase; optimization; central composite design;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A factorial design was performed to find the best conditions of pH and temperature for beta-xylanase activity and to maintain its activity for prolonged periods of time of pure xylanase produced by newly isolated Thermomyces lanuginosus THKU-49. The central composite design (CCD) used for the analysis of treatment combinations showed that a regression models of optimization of xylanase activity and xylanase stability were good agreement to experimental results with R-2 = 0.98 and 0.99, respectively. The maximum activity of xylanase was obtained at 66 degrees C and pH 6.3. The temperature and pH for maximum enzyme stability was 70 degrees C and pH 7.3, respectively. Under this condition xylanase having half-life of 825 min indicated the highest thermostable xylanase.
引用
收藏
页码：3599 / 3602
页数：4
相关论文
共 49 条
  • [1] Purification and characterization of a high-thermostable β-xylanase from newly isolated Thermomyces lanuginosus THKU-49
    Khucharoenphaisan, Khwanchai
    Tokuyama, Shinji
    Kitpreechavanich, Vichien
    MYCOSCIENCE, 2010, 51 (06) : 405 - 410
  • [2] Optimization of fibrinolytic enzyme production by newly isolated Bacillus subtilis Egy using central composite design
    Moharam, Maysa E.
    El-Bendary, Magda A.
    El-Beih, Fawkia
    Easa, Saadia M. Hassanin
    Elsoud, Mostafa M. Abo
    Azzam, Mohamed I.
    Elgamal, Nora N.
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2019, 17 : 43 - 50
  • [3] Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy
    Priyashini Dhaver
    Brett Pletschke
    Bruce Sithole
    Roshini Govinden
    Scientific Reports, 12
  • [4] Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy
    Dhaver, Priyashini
    Pletschke, Brett
    Sithole, Bruce
    Govinden, Roshini
    SCIENTIFIC REPORTS, 2022, 12 (01)
  • [5] Statistical Optimization of the Growth Factors for Chaetoceros neogracile Using Fractional Factorial Design and Central Composite Design
    Jeong, Sung-Eun
    Park, Jae-Kweon
    Kim, Jeong-Dong
    Chang, In-Jeong
    Hong, Seong-Joo
    Kang, Sung-Ho
    Lee, Choul-Gyun
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 18 (12) : 1919 - 1926
  • [6] Enhanced production of xylanase by a newly isolated Aspergillus terreus under solid state fermentation using palm industrial waste: A statistical optimization
    Lakshmi, Garapati Suvarna
    Rao, Chaganti Subba
    Rao, Ravella Sreenivas
    Hobbs, Phil J.
    Prakasham, Reddy Shetty
    BIOCHEMICAL ENGINEERING JOURNAL, 2009, 48 (01) : 51 - 57
  • [7] Valorization of de-oiled cakes for xylanase production and optimization using central composite design by Trichoderma koeningi isolate
    Bandikari, Ramesh
    Katike, Umamahesh
    Seelam, Naga Sivudu
    Obulam, Vijaya Sarathi Reddy
    TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, 2017, 42 (03): : 317 - 328
  • [8] Optimization of microalgae Scenedesmus SP. growth rate using a central composite design statistical approach
    Derakhshandeh, Masoud
    Un, Umran Tezcan
    BIOMASS & BIOENERGY, 2019, 122 : 211 - 220
  • [9] Optimization of xylanase production by Streptomyces sp P12-137 using response surface methodology and central composite design
    Coman, Gigi
    Bahrim, Gabriela
    ANNALS OF MICROBIOLOGY, 2011, 61 (04) : 773 - 779
  • [10] Optimization of xylanase production by Streptomyces sp. P12-137 using response surface methodology and central composite design
    Gigi Coman
    Gabriela Bahrim
    Annals of Microbiology, 2011, 61 : 773 - 779
12345