Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii

被引：50

作者：

Lorena Almaraz-Delgado, Alma ^{[1
]}

Flores-Uribe, Jose ^{[1
]}

Hugo Perez-Espana, Victor ^{[1
]}

Salgado-Manjarrez, Edgar ^{[1
]}

Agustin Badillo-Corona, Jesus ^{[1
]}

机构：

[1] Inst Politecn Nacl, Unidad Profes Interdisciplinaria Biotecnol, Mexico City 07340, DF, Mexico

来源：

AMB EXPRESS | 2014年 / 4卷

关键词：

Chlamydomonas; Chloroplast transformation; Recombinant therapeutic proteins; MOUTH-DISEASE VIRUS; SYNTHETIC BIOLOGY; EXPRESSION; TRANSFORMATION; ANTIBODY; MICROALGAE; VACCINES; PLANTS; TOOLS; ALGAE;

D O I：

10.1186/s13568-014-0057-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have been successfully expressed in the chloroplast using endogenous and chimeric promoter sequences. In some cases, proteins have accumulated to high level, demonstrating that this technology could compete with current production platforms. This review focuses on the works that have engineered the chloroplast of C. reinhardtii with the aim of producing recombinant proteins intended for therapeutical use in humans or animals.

引用

页码：1 / 9

页数：9

共 50 条

[31] REVERSE CHLOROPLAST GENETICS IN CHLAMYDOMONAS-REINHARDTII
ROCHAIX, JD
ZERGES, W
GOLDSCHMIDTCLERMONT, M
PHOTOSYNTHESIS RESEARCH, 1992, 34 (01) : 95 - 95
[32] Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii
Reifschneider-Wegner, Kiera
Kanygin, Andrey
Redding, Kevin E.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2014, 39 (08) : 3657 - 3665
[33] Intron-specific RNA binding proteins in the chloroplast of the green alga Chlamydomonas reinhardtii
Bunse, AA
Nickelsen, J
Kück, U
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2001, 1519 (1-2): : 46 - 54
[34] Co-translational import of nuclear-encoded proteins into the chloroplast in Chlamydomonas reinhardtii
Billakurthi, Kumari
Loudya, Naresh
PLANT PHYSIOLOGY, 2024, 196 (01) : 10 - 11
[35] TRYPTIC DIGESTION OF MEMBRANE-PROTEINS SYNTHESIZED ON CHLOROPLAST RIBOSOMES IN CHLAMYDOMONAS-REINHARDTII
MICHAELS, A
SEXTON, JT
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 610 (01) : 167 - 173
[36] Translational regulation of chloroplast genes proteins binding to the 5 prime -untranslated regions of chloroplast mRNAs in chlamydomonas reinhardtii
J. Biol. Chem., 3 (1486-1497):
[37] A NUCLEAR MUTATION AFFECTS THE SYNTHESIS OF THE CHLOROPLAST PSBA GENE PRODUCTION CHLAMYDOMONAS-REINHARDTII
GIRARDBASCOU, J
PIERRE, Y
DRAPIER, D
CURRENT GENETICS, 1992, 22 (01) : 47 - 52
[38] Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast
Yang, Zongqi
Li, Yinu
Chen, Feng
Li, Dong
Zhang, Zhifang
Liu, Yanxin
Zheng, Dexian
Wang, Yong
Shen, Guifang
CHINESE SCIENCE BULLETIN, 2006, 51 (14): : 1703 - 1709
[39] Translational regulation of chloroplast gene expression in Chlamydomonas reinhardtii
Cohen, A
Yohn, CB
Bruick, RK
Mayfield, SP
PHOTOSYNTHESIS: MOLECULAR BIOLOGY OF ENERGY CAPTURE, 1998, 297 : 192 - 208
[40] GLUCOSE RESPIRATION IN THE INTACT CHLOROPLAST OF CHLAMYDOMONAS-REINHARDTII
CHEN, CG
GIBBS, M
PLANT PHYSIOLOGY, 1991, 95 (01) : 82 - 87

← 1 2 3 4 5 →