Validation of a LC-fluorescence method for determination of free captopril in human plasma, using a pre-column derivatization reaction with monobromobimane

被引：42
|
作者：
Tache, F
Farca, A
Medvedovici, A
David, V
机构：
[1] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest 5, Romania
[2] Labormed Pharma SA, Bucharest 76246 5, Romania
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2002年 / 28卷 / 3-4期
关键词：
free captopril; human plasma; pre-column derivatization; monobromobimane; HPLC-FLD;
D O I：
10.1016/S0731-7085(01)00687-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Both derivatization of free captopril in human plasma samples using monobromobimane as fluorescent label and the corresponding HPLC-fluorescence detection (FLD) method were validated. Calibration curve for the fluorescent captopril derivative in plasma samples is linear in the ppb-ppm range with a detection limit of 4 ppb and an identification limit of 10 ppb (P%: 90; v greater than or equal to 5). These methods were successfully applied on bioequivalence studies carried out on some marketed pharmaceutical formulations. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：549 / 557
页数：9
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