A colorimetric assay for S-adenosylhomocysteine hydrolase

被引：32
|
作者：
Lozada-Ramirez, J. D. [1 ]
Martinez-Martinez, I. [1 ]
Sanchez-Ferrer, A. [1 ]
Garcia-Carmona, F. [1 ]
机构：
[1] Univ Murcia, Fac Biol, Dept Biochem & Mol Biol A, E-30071 Murcia, Spain
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2006年 / 67卷 / 2-3期
关键词：
S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine; homocysteine; DTNB; colorimetric assay;
D O I：
10.1016/j.jbbm.2006.01.008
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A colorimetric method for S-adenosyl-L-homocysteine hydrolase (SAHase) which uses S-adenosyl-L-homocysteine (SAH) as substrate is described. This method involves the hydrolytic conversion of SAH into adenosine (ADO) and L-homocysteine (HCY). The formation of HCY is quantified using Ellman's reagent and spectrophotometrical measured at 412 nm. Under these assay conditions, the product was followed continuously in a facile and quantitative manner until substrate conversion was complete. This method is an easy, cheap and shorter alternative to more complex methods and it is applicable to routine clinical analysis and in the assay and development of new S-nucleosidylhomocysteines to be used as therapeutic compounds. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：131 / 140
页数：10
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