Nakamurella silvestris sp nov., an actinobacterium isolated from alpine forest soil

A Gram-stain-positive, non-motile, catalase-positive and cytochrome c oxidase-negative bacterium, designated strain S20-107(T), was isolated from alpine forest soil. Growth occurred at 0-30 degrees C, at pH 6-9 and in the presence of 0-3% (w/v) NaCI. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S20-107T was related to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity with Nakamurella flavida DS-52(T) (96.1%). Strain S20-107(T) showed <96.1% 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella. The cell-wall peptidoglycan contained meso-diaminopinnelic acid. The major whole-cell sugars were glucose, galactose, mannose, arabinose, ribose and rhamnose. The strain contained MK-8(H-4) as the predominant menaquinone and diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminophospholipid as the major polar lipids. The major cellular fatty acids were anteiso-C-15:0, C-16:0, summed feature 3 (C-16:1 omega 6c and/or C-16:(1)omega 7c and/or iso-C-15:0 2-0H) and iso-C-16:0. The genomic DNA G+C content was 70.5 mol%. Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-107(T) represents a novel species of the genus Nakamurella, for which the name Nakamurella silvestris sp. nov. is proposed. The type strain is S20-107(T) (=DSM 102309(T)=LMG 29427(T)).