Adjacent intact nociceptive neurons drive the acute outburst of pain following peripheral axotomy

被引：13

作者：

Chen, Zhiyong ^{[1
,2
,3
]}

Wang, Tao ^{[1
,4
]}

Fang, Yehong ^{[1
,2
]}

Luo, Dan ^{[5
,6
]}

Anderson, Michael ^{[3
]}

Huang, Qian ^{[3
]}

He, Shaoqiu ^{[3
]}

Song, Xiaodan ^{[3
]}

Cui, Huan ^{[1
,2
]}

Dong, Xinzhong ^{[7
,8
,9
,10
]}

Xie, Yikuan ^{[1
,2
]}

Guan, Yun ^{[3
,9
]}

Ma, Chao ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Dept Human Anat Histol & Embryol, Sch Basic Med,Neurosci Ctr, Inst Basic Med Sci,Peking Union Med Coll, Beijing 100005, Peoples R China

[2] Peking Union Med Coll, Joint Lab Anesthesia & Pain, Beijing 100730, Peoples R China

[3] Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21205 USA

[4] Capital Med Univ, Xuanwu Hosp, Dept Neurosurg, Beijing 100053, Peoples R China

[5] Chinese Acad Med Sci, Inst Basic Med Sci, Natl Key Lab Med Mol Biol, Beijing 100005, Peoples R China

[6] Chinese Acad Med Sci, Inst Basic Med Sci, Dept Immunol, Beijing 100005, Peoples R China

[7] Harbin Med Univ, Coll Pharm, Harbin 150081, Heilongjiang, Peoples R China

[8] Johns Hopkins Univ, Sch Med, Solomon H Snyder Dept Neurosci, Ctr Sensory Biol, Baltimore, MD 21205 USA

[9] Johns Hopkins Univ, Sch Med, Dept Neurol Surg, Baltimore, MD 21205 USA

[10] Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Baltimore, MD 21205 USA

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

基金：

中国国家自然科学基金;

关键词：

BRACHIAL-PLEXUS INJURY; SPINAL NERVE LIGATION; NEUROPATHIC PAIN; RAT MODEL; AFFERENTS; FREQUENCY; SURGERY; DEMYELINATION; MECHANISMS; INHIBITION;

D O I：

10.1038/s41598-019-44172-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Injury of peripheral nerves may quickly induce severe pain, but the mechanism remains obscure. We observed a rapid onset of spontaneous pain and evoked pain hypersensitivity after acute transection of the L5 spinal nerve (SNT) in awake rats. The outburst of pain was associated with a rapid development of spontaneous activities and hyperexcitability of nociceptive neurons in the adjacent uninjured L4 dorsal root ganglion (DRG), as revealed by both in vivo electrophysiological recording and high-throughput calcium imaging in vivo. Transection of the L4 dorsal root or intrathecal infusion of aminobutyrate aminotransferase inhibitor attenuated the spontaneous activity, suggesting that retrograde signals from the spinal cord may contribute to the sensitization of L4 DRG neurons after L5 SNT. Electrical stimulation of low-threshold afferents proximal to the axotomized L5 spinal nerve attenuated the spontaneous activities in L4 DRG and pain behavior. These findings suggest that peripheral axotomy may quickly induce hyperexcitability of uninjured nociceptors in the adjacent DRG that drives an outburst of pain.

引用

页数：12

共 41 条

[41] Upregulation of N-type calcium channels in the soma of uninjured dorsal root ganglion neurons contributes to neuropathic pain by increasing neuronal excitability following peripheral nerve injury
Yang, Jie
Xie, Man-Xiu
Hu, Li
Wang, Xiao-Fang
Mai, Jie-Zhen
Li, Yong-Yong
Wu, Ning
Zhang, Cheng
Li, Jin
Pang, Rui-Ping
Liu, Xian-Guo
BRAIN BEHAVIOR AND IMMUNITY, 2018, 71 : 52 - 65

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