Structural Characterization of a Directed Self-Assembly Recombinant Quasi-Spider Silk Protein Expressed in Escherichia coli

被引：1
|
作者：
Liu, Bin [1 ]
Wang, Tao [1 ]
Xiao, Liyan [2 ]
Li, Yun [1 ]
Zhu, Min [1 ]
Lan, Wanqi [1 ]
Zhong, Wnsun [1 ]
Fu, Qiang [1 ]
机构：
[1] Jinggangshan Univ, Dept Med, Jian 343009, Jiangxi, Peoples R China
[2] Jinggangshan Univ, Sch Foreign Languages, Jian 343009, Jiangxi, Peoples R China
来源：
INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY | 2019年 / 21卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Collagen-like peptides; Directed self-assembly; Recombinant quasi-spider silk protein; Structure characterization; COLLAGEN;
D O I：
10.17957/IJAB/15.0953
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Native spider silk protein is an extracellular fibrous protein with distinctive characteristics of elasticity and strength. However, its application was limited for several shortcomings. Here we expressed a recombinant quasi-spider silk protein in which collagen-like peptides were fused to the spider silk protein in Escherichia coli. This recombinant protein is a "ABA" type triblock copolymer. Part A which located in both ends of the protein composed of (Pro-Gly-Pro)(n) homopolymeric stretches, formed the orientable collagen three-helix structure. While, the intervening block (part B) is the spider silk protein which is optimally designed. Results showed that the interest protein gene was successfully expressed in Escherichia coli. Structure characterization of this recombinant protein indicates tremendous potential usage in new biomedical material field. (C) 2019 Friends Science Publishers
引用
收藏
页码：750 / 756
页数：7
相关论文
共 50 条
  • [31] Immunological characterization of recombinant Salmonella enterica serovar Typhi FliC protein expressed in Escherichia coli
    Jindal, Gaurav
    Tewari, Rupinder
    Gautam, Ankur
    Pandey, Satish K.
    Rishi, Praveen
    AMB EXPRESS, 2012, 2
  • [32] Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli
    Pearce, Lesley A.
    Yu, Meng
    Waddington, Lynne J.
    Barr, Jennifer A.
    Scoble, Judith A.
    Crameri, Gary S.
    McKinstry, William J.
    PROTEIN EXPRESSION AND PURIFICATION, 2015, 116 : 19 - 29
  • [33] Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits
    Guo, Huimin
    Zhu, Jie
    Tan, Yonggui
    Li, Chuanfeng
    Chen, Zongyan
    Sun, Shiqi
    Liu, Guangqing
    ANTIVIRAL RESEARCH, 2016, 131 : 85 - 91
  • [34] Self-assembly and structural characterization of Echinococcus granulosus antigen B recombinant subunit oligomers
    Monteiro, Karina M.
    Scapin, Sandra M. N.
    Navarro, Marcos V. A. S.
    Zanchin, Nilson I. T.
    Cardoso, Mateus B.
    da Silveira, Nadya P.
    Goncalves, Paulo F. B.
    Stassen, Hubert K.
    Zaha, Arnaldo
    Ferreira, Henrique B.
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2007, 1774 (02): : 278 - 285
  • [35] Dimerization of the Conserved N-Terminal Domain of a Spider Silk Protein Controls the Self-Assembly of the Repetitive Core Domain
    Bauer, Joschka
    Scheibel, Thomas
    BIOMACROMOLECULES, 2017, 18 (08) : 2521 - 2528
  • [36] Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli
    Tan, HD
    Dan, GP
    Gong, HY
    Cao, LJ
    BIOTECHNOLOGY LETTERS, 2005, 27 (13) : 905 - 910
  • [37] Purification and Characterization of Recombinant Truncated Human Interleukin-11 Expressed as Fusion Protein in Escherichia coli
    Haidong Tan
    Guoping Dan
    Huiying Gong
    Lijun Cao
    Biotechnology Letters, 2005, 27 : 905 - 910
  • [38] Refolding and characterization of recombinant human GST-PD-1 fusion protein expressed in Escherichia coli
    Li, DW
    Yu, JF
    Chen, YJ
    Ma, HB
    Wang, ZF
    Zhu, YB
    Zhang, XG
    ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2004, 36 (02) : 141 - 146
  • [39] PURIFICATION AND CHARACTERIZATION OF RECOMBINANT SPINACH ACYL CARRIER PROTEIN-I EXPRESSED IN ESCHERICHIA-COLI
    GUERRA, DJ
    DZIEWANOWSKA, K
    OHLROGGE, JB
    BEREMAND, PD
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1988, 263 (09) : 4386 - 4391
  • [40] Purification, characterization, assembly and crystallization of assembled alfalfa mosaic virus coat protein expressed in Escherichia coli
    Yusibov, V
    Kumar, A
    North, A
    Johnson, JE
    LoeschFries, LS
    JOURNAL OF GENERAL VIROLOGY, 1996, 77 : 567 - 573
12345