Stopless separation of proteins by frit-inlet asymmetrical flow field-flow fractionation

The frit inlet asymmetrical flow field-flow fractionation is applied to the separation of proteins by using a stopless flow injection procedure. By utilizing a small permeable frit near the injection point in an asymmetrical flow FFF channel, sample materials injected to the flow streams can be hydrodynamically relaxed by the compressing action of high speed frit flow and the focusing/relaxation procedure can be bypassed. The separation efficiency of the frit inlet asymmetrical flow FFF channel is demonstrated with few protein standards by examining the influence of the ratio of injection flow rate to frit flow rate on the band broadening during relaxation.