Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative staphylococci and determine methicillin resistance from blood cultures

被引：168
|
作者：
Maes, N [1 ]
Magdalena, J [1 ]
Rottiers, S [1 ]
De Gheldre, Y [1 ]
Struelens, MJ [1 ]
机构：
[1] Free Univ Brussels, Hop Erasme, Microbiol Lab, Microbiol Serv,Ctr Mol Diagnost, B-1070 Brussels, Belgium
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 04期
关键词：
D O I：
10.1128/JCM.40.4.1514-1517.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for identification of staphylococci and detection of methicillin resistance. After validation of the assay with a collection of strains of staphylococci and enterococci (n = 169), the assay was evaluated with cultures of blood with gram-positive cocci from 40 patients. Accurate results were obtained for 59 (98%) of 61 cultures within 6 h of growth detection.
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页码：1514 / 1517
页数：4
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