Simultaneous Determination of Tramadol and Ibuprofen in Pharmaceutical Preparations by First Order Derivative Spectrophotometric and LC Methods

The simultaneous determination of tramadol (Trama) and ibuprofen (Ibu) in solid dosage forms were done by two methods. The first involved determination using the first order derivative spectrophotometric technique at 230.5 and 280 nm over the concentration ranges of 5-50 and 5-100 mu g mL-1 with mean accuracies 99.84 and 99.98%, respectively. The second method was reversed-phase liquid chromatography using chlorzoxazone (Cxz) as an internal standard. Analysis was carried out using acetonirile-0.1% acetic acid (60 + 40, v/v) as the mobile phase with detection at 272 nm for Trama, Cxz and at 230 nm for Ibu with flow rates of 1 mL min(-1) and 1.3 mL min(-1), respectively. The retention times for Trama, Cxz and Ibu were 2.14, 4.10 and 9.04, respectively. The mean recovery of Trama and Ibu was 99.91 and 99.92%. Due to its simplicity, rapidness, high precision and accuracy, the proposed methods can be used for the estimation of tramadol and ibuprofen in pharmaceutical preparations.