Biological Compound Capping of Silver Nanoparticle with the Seed Extracts of Blackcumin (Nigella sativa): A Potential Antibacterial, Antidiabetic, Anti-inflammatory, and Antioxidant

It is a well explored facts that need of biosynthesized nanomaterials has emerged for its several advantages in biomedical applications and the search for potential biosynthesized-nanomaterials is still ongoing. In this study an attempt was made to meet the requirement of potential biosynthesized-silver nanoparticles using the aqueous seed extract ofN. sativa(Bc-AgNPs). X-ray powder diffraction (XRD) confirms the biosynthesis of silver nanoparticle (supports the observation of UV-Vis spectroscopy) with face centred cubic structure. Fourier-transform infrared spectroscopy (FTIR) confirms the capping of the active ingredients of seed extract and further the average size of 34 nm was confirmed by high-resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray analysis (EDX) confirms the purity of Bc-AgNPs. Due to the capping of the active ingredients of seed extract, the Bc-AgNPs have shown significant antibacterial activity against Gram negative bacteria and at the lower concentration of 100 mu g*ml(-1)it has 89% scavenging activity against free radicals. A strong anti-inflammatory (membrane stabilization) activity towards RBCs (98% at 100 mu g*ml(-1)) was recorded when compared to the positive control (aceclofenac). In addition to the previous activities, a greater inhibition of carbohydrate hydrolyzing enzymes such as alpha-amylase, alpha-glucosidase and DPP-IV highlighted the antidiabetic potential of these green synthesized AgNPs. Overall, the efficient bioactivities of Bc-AgNPs advocates its future potential in biomedical products.