Label-free Detection of Single Nucleotide Polymorphism Using Selective Incorporation of Biotin Anchor and Magnetoseperation on Streptavidin Magnetic Beads

被引：0

作者：

Plucnara, Medard ^{[1
]}

Havran, Ludek ^{[1
]}

Ecsin, Ece ^{[2
]}

Erdem, Arzum ^{[2
]}

Fojta, Miroslav ^{[1
]}

机构：

[1] Inst Biophys AS CR, Vvi, Kralovopolska 135, Brno 61265, Czech Republic

[2] Ege Univ, Fac Pharm, Analyt Chem Dept, TR-35100 Bornova, Izmir, Turkey

来源：

XXXVI MODERNI ELEKTROCHEMICKE METODY | 2016年

关键词：

Label-free detection; Single nucleotide polymorphism; Pencil graphite electrode; SURFACE-PLASMON RESONANCE; COPPER IONS;

D O I：

暂无

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Novel approach of SNP detection in model synthetic oligonucleotides is described.This assay requires only intrinsic electrochemical properties of DNA and except of incorporation of biotin affinity tag, no any labeling or any other modification step is needed. Very good resolution for different variants of polymorphism as well as sufficiently low detection limits were achieved.In combination with using pencil graphited electrode this assay could be very simple and cheap tool for SNP typing.

引用

页码：161 / 165

页数：5

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