Genetic Variability of CSN1S1 Gene in Goat Populations Raised in Southeastern Region of Turkey

被引:1
|
作者
Bozkaya, Faruk [1 ]
Gurler, Sukru [1 ]
Yerturk, Mugdat [2 ]
机构
[1] Harran Univ, Fac Vet Med, Dept Genet, TR-63200 Sanliurfa, Turkey
[2] Yuzuncu Yil Univ, Fac Vet Med, Dept Zootech, TR-65080 Van, Turkey
关键词
Goat; Casein; CSN1S1; Polymorphism; POLYMERASE-CHAIN-REACTION; COAGULATION PROPERTIES; BETA-LACTOGLOBULIN; MILK-COMPOSITION; PCR-RFLP; AS-PCR; POLYMORPHISM; ALPHA(S1)-CASEIN; ALLELE; ALPHA-S1-CASEIN;
D O I
10.9775/kvfd.2012.7402
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The objective of this study was to investigate genetic variability of CSN1S1 gene coding for alpha-s1-casein in goat populations raised in Southeastern Region of Turkey. Blood samples were collected from goats raised in Kilis(n=60), Sanliurfa (n=56), and Siirt (n=55) provinces of Turkey. From the blood samples DNA was isolated by using phenol-chloroform extraction. Genotypes of animals were determined by using polymerase chain reaction (PCR), allele specific PCR or PCR and restriction fragment length polymorphism methods. In Kilis and Sanliurfa populations CSN1S1 A*, B*, F and N alleles were observed, while in Siirt population only A* and B* alleles were found. Frequencies of A*, B*, F and N alleles were 0.375, 0.367, 0.017 and 0.242 in Kills, 0.632, 0.208, 0.009 and 0.151 in Sanliurfa and 0.782, 0.218, 0.000 and 0.000 in Siirt populations, respectively. CSN1S1 E and 01 alleles were not observed among the populations studied. Observed and expected genotype frequencies did not differ significantly (P>0.05). The results of this study suggested that there were sufficient genetic variability of CSN1S1 gene especially in Sanliurfa and Kilis populations in order to select individuals for different breeding purposes.
引用
收藏
页码:147 / 152
页数:6
相关论文
共 50 条
  • [21] Genetic Diversity of Eight Domestic Goat Populations Raised in Turkey
    Bulut, Zafer
    Kurar, Ercan
    Ozsensoy, Yusuf
    Altunok, Vahdettin
    Nizamlioglu, Mehmet
    BIOMED RESEARCH INTERNATIONAL, 2016, 2016
  • [22] Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey
    Gurler, Sukru
    Bozkaya, Faruk
    KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, 2013, 19 (02) : 207 - 213
  • [23] Mediterranean river buffalo CSN1S1 gene: search for polymorphisms and association studies
    Cosenza, G.
    Pauciullo, A.
    Macciotta, N. P. P.
    Apicella, E.
    Steri, R.
    La Battaglia, A.
    Jemma, L.
    Coletta, A.
    Di Berardino, D.
    Ramunno, L.
    ANIMAL PRODUCTION SCIENCE, 2015, 55 (05) : 654 - 660
  • [24] A Single Nucleotide Polymorphism and Sequence Analysis of CSN1S1 Gene Promoter Region in Chinese BOS Grunniens (YAK)
    Bai, W. L.
    Yin, R. H.
    Dou, Q. L.
    Yang, J. C.
    Zhao, S. J.
    Ma, Z. J.
    Yin, R. L.
    Luo, G. B.
    Zhao, Z. H.
    ANIMAL BIOTECHNOLOGY, 2010, 21 (01) : 36 - 41
  • [25] Genomics and proteomics of deleted ovine CSN1S1*I
    Giambra, Isabella Jasmin
    Chianese, Lina
    Ferranti, Pasquale
    Erhardt, Georg
    INTERNATIONAL DAIRY JOURNAL, 2010, 20 (03) : 195 - 202
  • [26] Determination the effect of CSN1S1, CSN3 and AGPAT6 genes and lactation rank on physicochemical properties of goat milk
    Deniz, Dincel
    Sena, Ardicli
    Hale, Samli
    Faruk, Balci
    LARGE ANIMAL REVIEW, 2020, 26 (04): : 167 - 171
  • [27] Negative regulation of αS1-casein (CSN1S1) improves β-casein content and reduces allergy potential in goat milk
    Song, Ning
    Chen, Yating
    Luo, Jun
    Huang, Lian
    Tian, Huibin
    Li, Cong
    Loor, Juan J.
    JOURNAL OF DAIRY SCIENCE, 2020, 103 (10) : 9561 - 9572
  • [28] A trinucleotide repeat polymorphism is present in bovine CSN1S1
    Prinzenberg, EM
    Hiendleder, S
    Erhardt, G
    ANIMAL GENETICS, 1997, 28 (05) : 379 - 380
  • [29] 5’-flanking variants of equine casein genes (CSN1S1, CSN1S2, CSN2, CSN3) and their relationship with gene expression and milk composition
    Jakub Cieslak
    Lukasz Wodas
    Alicja Borowska
    Piotr Pawlak
    Grazyna Czyzak-Runowska
    Jacek Wojtowski
    Kamila Puppel
    Beata Kuczynska
    Mariusz Mackowski
    Journal of Applied Genetics, 2019, 60 : 71 - 78
  • [30] 5'-flanking variants of equine casein genes (CSN1S1, CSN1S2, CSN2, CSN3) and their relationship with gene expression and milk composition
    Cieslak, Jakub
    Wodas, Lukasz
    Borowska, Alicja
    Pawlak, Piotr
    Czyzak-Runowska, Grazyna
    Wojtowski, Jacek
    Puppel, Kamila
    Kuczynska, Beata
    Mackowski, Mariusz
    JOURNAL OF APPLIED GENETICS, 2019, 60 (01) : 71 - 78