Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli

被引：32
|
作者：
Shi, Yanxiang
Bueno, Alejandro
van der Donk, Wilfred A. [1 ]
机构：
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
来源：
CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 89期
基金：
美国国家卫生研究院;
关键词：
PEPTIDOGLYCAN SYNTHESIS; BIOSYNTHESIS; ACTAGARDINE; MODE; MONOOXYGENASES; ORGANIZATION;
D O I：
10.1039/c2cc36336d
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.
引用
收藏
页码：10966 / 10968
页数：3
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