High-dimensional and large-scale phenotyping of yeast mutants

被引：219
|
作者：
Ohya, Y
Sese, J
Yukawa, M
Sano, F
Nakatani, Y
Saito, TL
Saka, A
Fukuda, T
Ishihara, S
Oka, S
Suzuki, G
Watanabe, M
Hirata, A
Ohtani, M
Sawai, H
Fraysse, N
Latgé, JP
François, JM
Aebi, M
Tanaka, S
Muramatsu, S
Araki, H
Sonoike, K
Nogami, S
Morishita, S
机构：
[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Kashiwa, Chiba 2778562, Japan
[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Computat Biol, Kashiwa, Chiba 2778562, Japan
[3] Japan Sci & Technol Corp, Inst Bioinformat & Res & Dev, Chiyoda Ku, Tokyo 1028666, Japan
[4] Univ Tokyo, Dept Comp Sci, Grad Sch Informat Sci & Technol, Bunkyo Ku, Tokyo 1130033, Japan
[5] Inst Pasteur, Unite Aspergillus, F-75015 Paris, France
[6] CNRS, Ctr Bioingn Gilbert Durand, UMR 5504, INRA, F-31077 Toulouse, France
[7] ETH, ETH Honggerberg, Inst Microbiol, CH-8093 Zurich, Switzerland
[8] Natl Inst Genet, Div Microbial Genet, Mishima, Shizuoka 4118540, Japan
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 52期
关键词：
cell morphology; functional genomics; high-dimensional phenotyping; phenome;
D O I：
10.1073/pnas.0509436102
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.
引用
收藏
页码：19015 / 19020
页数：6
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