Proteins in the insulin-secreting cell line MIN6 bind the imidazoline compound BL11282

被引：2

作者：

Shafqat, Jawed

Ishrat, Moin

Jagerbrink, Theres

Sillard, Rannar

Maeorg, Uno ^{[3
]}

Efendic, Suad

Berggren, Per-Olof

Zaitsev, Sergei V. ^{[2
]}

Jornvall, Hans ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, Rolf Luft Ctr Diabet & Endocrinol, Stockholm, Sweden

[2] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow, Russia

[3] Univ Tartu, Inst Chem, EE-50090 Tartu, Estonia

来源：

FEBS LETTERS | 2008年 / 582卷 / 11期

关键词：

imidazoline compounds; surface plasmon resonance; MIN6; cells; elongation factor 1A; glucose regulated protein 94;

D O I：

10.1016/j.febslet.2008.04.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282-labelled magnetic beads. Bound material extracted from the beads showed a similar to 50 kDa differential band upon SDS-PAGE and a weaker 100 kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the similar to 100 kDa band. Masspectrometric analysis revealed the similar to 50 kDa band to be EF1A and the similar to 100 kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1613 / 1617

页数：5

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