High-Throughput Microfluidic Device for Rare Cell Isolation

被引：5

作者：

Yang, Daniel ^{[1
]}

Leong, Serena ^{[2
]}

Lei, Andy ^{[2
]}

Sohn, Lydia L. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

来源：

BIO-MEMS AND MEDICAL MICRODEVICES II | 2015年 / 9518卷

关键词：

CIRCULATING TUMOR-CELLS; WHOLE-BLOOD; SEPARATION;

D O I：

10.1117/12.2178613

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Enumerating and analyzing circulating tumor cells (CTCs)-cells that have been shed from primary solid tumors-can potentially be used to determine patient prognosis and track the progression of disease. There is a great challenge to create an effective platform that can isolate these cells, as they are extremely rare: only 1-10 CTCs are present in a 7.5mL of a cancer patient's peripheral blood. We have developed a novel microfluidic system that can isolate CTC populations label free. Our system consists of a multistage separator that employs inertial migration to sort cells based on size. We demonstrate the feasibility of our device by sorting colloids that are comparable in size to red blood cells (RBCs) and CTCs.

引用

页数：7

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